Scientific Program

Keynote Speaker

Devika Chithrani
University of Victoria, Canada
Title: “Smart” nanomaterials for cancer therapy

Biography: Dr. Devika Chithrani is an assistant professor in the Department of Physics and Astronomy at University of Victoria and also part of the medical physics faculty in British Columbia Cancer Agency, Victoria, Canada. She is also an iBESTresearch scientist at Li KaShing Knowledge institute, St. Michael hospital, Toronto. She was a recipient of the prestigious Natural Sciences and Engineering Research Council (NSERC) doctoral award and NSERC post-doctoral award during her graduate and post-graduate studies at university of Toronto, Canada. She was the recipient of the Physics Gold medal and Faculty Gold medal at her undergraduate convocation.

Abstract: Smart nanomaterials for cancer therapy: Recent developments in nanotechnology has provided new tools for cancer therapy and diagnosis. The technology is expected to generate innovations and play a critical role in cancer therapeutics. Among other nanoparticle (NP) systems, there has been tremendous progress made in the use of spherical gold NPs (GNPs), gold nanorods (GNRs), gold nanoshells (GNSs) and gold nanocages (GNCs) in cancer therapeutics. In treating cancer, radiation therapy and chemotherapy remain the most widely used treatment options and recent developments in cancer research show that the incorporation of gold nanostructures into these protocols has enhanced tumor cell killing. These nanostructures further provide strategies for better loading, targeting, and controlling the release of drugs to minimize the side effects of highly toxic anticancer drugs used in chemotherapy and photodynamic therapy. In addition, the heat generation capability of gold nanostructures upon exposure to UV or near infrared light is being used to damage tumor cells locally in photothermal therapy. Hence, gold nanostructures provide a versatile platform to integrate many therapeutic options leading to effective combinational therapy in the fight against cancer. In this lecture, the recent progress in the development of gold-based NPs towards improved therapeutics will be discussed. A multifunctional platform based on gold nanostructures with targeting ligands, therapeutic molecules, and imaging contrast agents, holds an array of promising directions for cancer research


Properties of Biomaterials

Session Introduction

Hnawi Salma Kaotar
Laboratoire LN2E, Faculté des Sciences, Morocco.
Title: Structural and chemical investigations of chicken feathers

Biography: Hnawi Salma Kaotar is from Laboratoire LN2E, Faculté des Sciences, Morocco.

Abstract: In this work, the main objective is to study the structural and chemical properties of chicken feathers in order to improve recovery of agro-food waste andidentify a most suitable process to extract keratin which is an important ingredient for cosmetic products. A chemical treatment of the chicken feathers was considered to bring out the content of the organic and mineral matters using the combustion technique also the hydrolysis of keratin.We have investigated the Scanning electron microscope which reveals a low level of carbon and high level of minerals compared to the initial material. These preliminary studies give chemical analysis of chicken feathers in carbon and other mineral elements. Keywords: Bio-polymer; agro-food waste; Poultry feather; nano biomaterials


Dr. Sikander Ali
Institute of Industrial Biotechnology, GC University Lahore, Pakistan
Title: Immobilization of a thermophilic manganese peroxidase fromAspergillus versicolor IMPP-1175 for the degradation of aromatics in textile effluent

Biography: Dr. Sikander Ali did his Ph.D (2005) from University of the Punjab, Lahore. He is an eminent researcher in Fermentation Technology. He has published over 185 research papers in Journals of National and International repute. As PI/Co-PI, he headed 3 National and 2 International research projects. He has supervised 66 MSc/BSc Hons, 40 MPhil and 2 Ph.Dresearch students. He has availed Fulbright Scholarship (2002-2003). His research output has been recognized by Frost and Sullivan (2003). He also got training in bioenzyme preparation from China (2004). In 2005, he was awarded with MEXT Scholarship, Japan. In 2006, he was declared as Active Scientist by PSF. He was honored with Endeavour Research Award (2011) for Post-Doc in Australia. He has been declared as the Productive Scientist of Pakistan by PCST (2002-2013). In 2014, Eminent Scientific Discovery acknowledged his contribution in Biomedical Research. He has participated in about 50 conferences/symposia/workshops

Abstract: The present investigation deals with the production of a thermophilic peroxidase by Aspergillus versicolour IMPP-1175 and enzyme immobilization by entrapment in calcium alginate to degrade aromatic compounds in textile waste water. The batch culture experiments were carried out using pre-treated cotton seed meal (CSM) as a raw substrate under solid-state-fermentation (SSF) in Erlenmeyer flasks (250 ml). Cultural conditions including 30 ml of saline water (pH 7.57) at 1:3 substrate to moisture ratio, time of incubation (48 h) and size of inoculum (8%, v/v) were optimized for the improved production (67.1 U/g) of manganese peroxidase (MnP). Metallurgical microscopy of unfermented and fermented CSM exhibited quite different surface properties of the particles. The crude MnP extract was then partially characterized to determine the thermophilic behaviour of the enzyme. The activity increased by 1.5 fold at 50ºC when incubated for 45 min. Hydrogen peroxide (1 M) was selected as an enzyme inducer in the assay mixture. Associative enzymes particularly catalase, laccase and lignin peroxidase were also observed, but their highest activities were 19.72, 4.28 and 31.45 U/g, respectively. The enzyme was partially purified by (NH4)2SO4 precipitation and maximum specific activity (2.028 U/mg) was detected at 60% salt concentration. The fold purification was found to be 2.088. During sample dialysis, the pellet was found to be superior compared to the supernatant. The enzyme MnP was lyophilized by a freeze dryer at –40ºC under vacuum. Notably over 2.1 fold highly active enzyme concentration was accomplished in only 15 min. A higher stability and activity of MnP was observed when 1.5 ml enzyme extract was immobilized by entrapment in 3% (w/v) calcium alginate for 25 min. The potential residual enzyme activity by the immobilized MnP was found to be 226.18 U/g which supported maximum degradation of 10% phenol solution. At 50% diluted sample of textile wastewater, 179.6 U/g of the immobilized MnP were consumed while the remaining activity declined sharply to 46.58 U/g showing optimal enzyme application. During the course of enzymatic reaction, the initial and final pH values were recorded 8.11 and 8.02, respectively. Molecular identification of the fungal strain Aspergillus versicolor IMPP-1175 showed 97% homology with Aspergillus species.


Elif Bedir
Koc University, Surface Science and Technology Center (KUYTAM),Turkey
Title: Microstructural and Biocompatibility Evolution of TiTaHfNbZr Coating Deposited by PVD Method

Biography: Elif Bedir is from Koc University, Surface Science and Technology Center (KUYTAM),Turkey

Abstract: Although the bulk properties of the biomaterial are critical determinants of the biological performance of the material, it is rare that a biomaterial with suitable bulk properties also possesses appropriate surface characteristics for clinical applications, and very few surfaces are truly biocompatible. In spite of ever-increasing knowledge about the surface modification methods or coating materials for implants, finding reliable coating for most metallic biomaterials, especially NiTi alloys, has not completed yet. Finding a biocompatible and durable coating for NiTi alloys, which has a high tendency to release allergic Ni atoms in biological environments, is a challenging task for researchers.


Advanced Materials and Biomaterials in Delivery Systems

Session Introduction

Dr.Wang Fengjun
Department of Otolaryngology, Head and Neck Surgery, Xiangya Hospital, Central South University
Title: Optimizing the property of nano-hydroxyapatite genevectors via variety of surface-modification

Biography: Dr.Wang Fengjun,MedicineDoctor (Ph.D, MD) in otorhinolaryngology, He gothis MD and Ph.Ddegree at XiangyaHospitalin 2014.nowheis aattending doctorin the department ofOtolaryngology Head & Neck Surgery, XiangyaHospital, Central South University.

Abstract: The aim of thisstudywas to synthesize a HAP genevectorwithlowercytotoxicity and higher transfection efficiency.The hydroxyapatite (HAP)nanoparticlesweresynthesizedthroughchemicalprecipitation and hydrothermal methods,PEG-PEI copolymersweresynthesized by conjugatingmethoxypoly(ethylene glycol) (mPEG) to branchedpolyethylenimine (PEI), then HAP were surface modifiedwithdifferent ratio of PEI or PEI-PEG. PEG-PEI-HAP/DNA/PEI-PEG mixtures werepreparedwithdifferentweight ratio of HAP/DNA via ultrasonic dispersion and mechanicalstirring. After the examination of complex size, zetapotential, cytotoxicity and DNA binding ability of the newlydevelopedcopolymers, itstransfectionefficiencywerechecked. Thesenovel double coatedvector complexes canrender high transfection efficiency in 293t cells. As the concentration of the second coating and the proportion of the grafting PEG varied, the transfection efficiencychangedaccordingly, of which the highestefficiencyachieved 61.5% by the PEI25K-PEG2K–HAP–DNA-PEI25K-PEG2K nano-vectorcomplex, whichis close to that (69.3%) of the control group of liposome.Thesenovelinorganicvectorsystemswithadequate transfection efficiency, biocompatibility and high carryingcapacity of loadtherapeuticgenecouldpossibly serve as the substitute products for the commercial liposomes.


Prof. Alexander Archakov
Institute of Biomedical Chemistry, Moscow, Russian Federation
Title: PHOSPHOLIPID MICELLES AS THEMEDICINES THEMSELVES AND DRUG DELIVERY SYSTEM

Biography: Phospholipids, especially phosphatidylcholine, are very commonly used in medicine as a drug delivery systems:most investigated of them are liposomes.The aim of work was the use of phospholipid micelles rather liposomes as drug delivery systems and as drugs themselves.To obtain the phospholipid micelles are extremely small size we used homogenization under high pressure, ultrafiltration and freeze-drying.Phosphoglivis Russian original drug, which includes the phospholipid micelles with a size of 30-50 nm in diameter with incorporated glycyrrhizinic acid,which possesses weak detergent properties and the ability to induce the synthesis of ƴ-interferon was used forthe treatment of liver diseases including viral hepatitis (B and C). Phosphogliv exists on pharmaceutical Russian market with volume of ~ 30 millions of dollars. Nowdays the other phospholipids micelles with size of 15-25 nm in diameter without glycyrrhizinicacid were produced for improvement of reverse cholesterol transport and normalization of lipid metabolism. Phospholipid micelles as drug delivery system are biodegradable, biologically inert, do not cause allergic, antigenic, or pyrogenic reaction. The new technology was created to produce phospholipid micelles with particles diameter 15-25 nm, in the form of lyophilic powder, which is stable at storage. The main principles of incorporation of pharmacologicaly active substances such as doxorubicine.arbidole, rifampicine etc. into phospholipid micelles considerably increased their bioavailability and therapeutic efficiency

Abstract: Phospholipids, especially phosphatidylcholine, are very commonly used in medicine as a drug delivery systems:most investigated of them are liposomes.The aim of work was the use of phospholipid micelles rather liposomes as drug delivery systems and as drugs themselves.To obtain the phospholipid micelles are extremely small size we used homogenization under high pressure, ultrafiltration and freeze-drying.Phosphoglivis Russian original drug, which includes the phospholipid micelles with a size of 30-50 nm in diameter with incorporated glycyrrhizinic acid,which possesses weak detergent properties and the ability to induce the synthesis of ƴ-interferon was used forthe treatment of liver diseases including viral hepatitis (B and C). Phosphogliv exists on pharmaceutical Russian market with volume of ~ 30 millions of dollars. Nowdays the other phospholipids micelles with size of 15-25 nm in diameter without glycyrrhizinicacid were produced for improvement of reverse cholesterol transport and normalization of lipid metabolism. Phospholipid micelles as drug delivery system are biodegradable, biologically inert, do not cause allergic, antigenic, or pyrogenic reaction. The new technology was created to produce phospholipid micelles with particles diameter 15-25 nm, in the form of lyophilic powder, which is stable at storage. The main principles of incorporation of pharmacologicaly active substances such as doxorubicine.arbidole, rifampicine etc. into phospholipid micelles considerably increased their bioavailability and therapeutic efficiency.


Dr. Joel I. Osorio, MD
RegenerAge International. Westhill University School of Medicine, MX 905610, Mexico
Title: Therapeutic effects of combinatorial biologics (Bioquantine®) and Spinal cord stimulation system on a patient with Spinal Cord section.

Biography: Dr. Joel I. Osorio, MD, is from RegenerAge International. Westhill University School of Medicine, MX 905610, Mexico.

Abstract: As it has been previously demonstrated that coelectroporation of Xenopus laevis frog oocytes with normal cells and cancerous cell lines induces the expression of pluripotency markers, and in experimental murine model studies that Bioquantine® extract (purified from intra- and extra-oocyte liquid phases of electroporated oocytes) showed potential as a treatment for a wide range of conditions as Squint, Spinal Cord Injury (SCI) and Cerebral Palsy among others. The current study observed beneficial changes with Bioquantine® administration in a patient with a severe SCI. Pluripotent stem cells have therapeutic and regenerative potential in clinical situations CNS disorders even cancer.2-3-7 One method of reprogramming somatic cells into pluripotent stem cells is to expose them to extracts prepared from Xenopus laevis oocytes1 We showed previously that coelectroporation of Xenopus laevis frog oocytes; with normal cells and cancerous cells lines, induces expression of markers of pluripotency.4 We also observed therapeutic effects of treatment with a purified extract (Bioquantine) of intra- and extra-oocyte liquid phases derived from electroporated X. laevis oocytes, on experimentally induced pathologies including murine models of melanoma, traumatic brain injury, and experimental skin wrinkling induced by squalenemonohydroperoxide (Paylian et al, 2016). The positive human findings for Spinal Cord Injury, and Cerebral Palsy with the results from previous animal studies with experimental models of traumatic brain injury, respectively (Paylian et al, 2016). Because of ethical reasons, legal restrictions, and a limited numbers of patients, we were able to treat only a very small number of patients. These results indicate that Bioquantine ® may be safe and well tolerated for use in humans, and deserves further study in a range of degenerative disorders. We propose that the mechanism of action of Bioquantine® in these various diseases derives from its unique pharmacology and combinatorial reprogramming properties. In conclusion, these preliminary findings suggest that Bioquantine is safe and well tolerated on patients with Cerebral Palsy and- Spinal Cord Injury, among others. In addition to the regenerative therapy and due to the patient condition, we decided to include the Restore- Sensor SureScan5-6 . Based on the of electrical stimulation for rehabilitation and regeneration after spinal cord injury published by Hamid and MacEwan 8-9 , we designed an improved delivery method for the in situ application of MSCs and Bioquantine® in combination with the RestoreSensor® SureScan® Conclusions: To the present day the patient who suffered a total section of spinal cord at T12-L1 shows an improvement in sensitivity, strength in striated muscle and smooth muscle connection, 9 months after the first therapy of cell regeneration and 1 month after the placement of RestoreSensor® at the level of the lesion, the patient with a complete medullary section shows an evident improvement on his therapy of physical rehabilitation in standing for the first time and showing a progressively important functionality.


Prof Takao Hanawa
Tokyo Medical and Dental University, Japan.
Title: New alloys and surfaces for next generation implants

Biography: Dr. Takao HANAWA is Professor of Department of Metallic Biomaterials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University since 2004. He was a president of the Japanese Society for Biomaterials and is now a member of Science Council of Japan. He has experienced as Deputy-in-General of Biomaterials Research Center, National Institute for Materials Science. He has developed several new metallic biomaterials and is now operating several research projects on metal-based medical and dental materials. In particular, zirconium alloys showing low magnetic susceptibility to decrease MRI artifact and nano-topography to accelerate the differentiation of stem cells by femto-second laser.

Abstract: Over 70% of implant devices are made of metals, because of their high strength, toughness, and durability. Metals are generally superior to ceramics and polymers on the above properties for medical devices. Therefore, it is difficult to replace metals in medical devices with ceramics or polymers. In this regard, research and development of metals continue on the purpose of improving mechanical and surface properties, which govern their mechanical and interfacial compatibility. Research and development of metals continue with the purpose of improving mechanical and surface properties, which govern their mechanical and tissue compatibility. After micro arc oxidation (MAO), bone formation on titanium is accelerated. Silver is easily contained in the surface oxide layer by the addition of silver in the electrolyte for MAO. In this case, live bacteria were killed and simultaneously calcification occurred in a certain silver amount. This result reveals that bone formation and antibacterial property are simultaneously performed by addition of calcium, phosphate, and silver to surface oxide layer by MAO. It is possible to obtain and control dual-functional property by this technique. To clarify the effects of micron/nano hybrid topography on cell behavior and morphology, we investigated the adhesion of human mesenchymal stem cell (hMSC) to titanium surfaces with three different topographies; namely, Micron, Nano, and Hybrid grooves which created using a femtosecond laser. In addition, immune-fluorescent detection of the differentiation of hMSC cultured on specimens after differentiation was conducted. Four cell types, neurocytes, adipocytes, osteocytes, and chondrocytes, were differentiated from hMSC. The different surface features had different effects on the differentiation of hMSC. In particular, the Hybrid surface topography promoted the osteogenic differentiation and chondrogenic differentiation. On the other hand, we have developed Zr-based alloys, showing low magnetic susceptibility, high strength and corrosion resistance, to decrease MRI artifact. A large-amount melting of Zr-1mass%Mo alloy and investigated mechanical properties, crystal phase, and magnetic susceptibility, for the commercialization of the alloy was performed. Finally, MRI artifact of spinal instruments consisting of the alloy and implanted in sheep spine was observed. 3T MRI artifact of spinal instruments consisting of the alloy and implanted into sheep spine occupied in 2 mm and the vertebral canal appeared. Therefore, this alloy is a candidate of MRI compatible alloy.


Tissue Engineering and Regenerative Medicine

Session Introduction

SteliosT. Andreadis
University at Buffalo, State University of New York
Title: Stem Cell Aging and Reprogramming: ImplicationsforRegenerative Medicine

Biography: Stelios T. Andreadis received his M.S. (Applied Mathematics) and Ph.D. degree in Chemical Engineering from the University of Michigan studying the dynamics of retroviral gene transfer for gene therapy. He then pursued postdoctoral training at the Center for Engineering in Medicine at Harvard Medical School, where he worked in the areas of gene therapy, tissue engineering and regenerative medicine. Currently he serves as Professor and Chair of Chemical and Biological Engineering, Professor of Biomedical Engineering and Member of the Center of Excellence in Bioinformatics and Life Sciences at the University at Buffalo, State University of New York. He is also the Director of the Stem Cells in Regenerative Medicine (SCiRM) Training Program that was recently funded by NYSTEM to train students in stem cell biology and bioengineering and applications of stem cells in regenerative medicine.

Abstract: Cardiovascular disease is the leading cause of mortality worldwide. Regarded as the therapeutic gold standard, treatment with autologous grafts suffers from several technical and patient-related risks. Tissue engineered small diameter blood vessels mayprovide a promising alternative solution as replacement grafts. In this study, weemployed adult and induced pluripotent stem cells to engineer fully functional vascular grafts that were implanted into the arterial circulation of a physiologically relevant ovine animal model, where they remained patent and underwent successful remodeling. During the course of these studies we observed that mesenchymal stem cells (MSC) originating from older donors suffer from limited proliferative capacity and significantly reduced myogenic differentiation potential. This is a major concern, as the patients most likely to suffer from cardiovascular disease are elderly and are in need of vascular grafts. Notably, we discovered that delivery of a single pluripotency associate transcription factor, Nanog reversed the proliferation and differentiation potential of MSC from adult donors. I will present data supporting this claim and our efforts to understand the mechanism of how Nanog promotes myogenic differentiation, contractile functionand extracellular matrix synthesis of senescent MSC. In addition to MSC, we recently discovered that Nanog restored the ability of senescent skeletal muscle cells (SkMC) to form myotubes, suggesting that molecular engineering strategies can reverse the effects of organismal aging and restore the potential of adult stem cellsfor use in cellular therapies and tissue regeneration.In the second part of my presentation I will focus on reprogramming of skin cells to neural crest stem cells (NC) and their derivatives. NC cells are induced by signaling events at the neural plate border during development of vertebrate embryos. Initially arising within the central nervous system, NC cells subsequently undergo an epithelial to mesenchymal transition to migrate into the periphery, where they differentiate into diverse cell types. We discovered that postnatal human epidermal keratinocytes (KC) can be reprogrammed toward the NC fate without genetic modification or reprogramming to the pluripotent state. Genome-wide transcriptome analyses show that KC-derived NC cells are similar to NC cells derived from human embryonic stem cells. Moreover, KC-NC give rise in vitro and in vivo to NC derivatives such as peripheral neurons, melanocytes, Schwann cells and mesenchymal cells (osteocytes, chondrocytes, adipocytes, and smooth muscle cells). Lineage tracing studies by implantation of KC-NC into chick embryos confirmed the NC phenotype of these cells. This work represents a paradigm shift as it demonstrates the plasticity of human epidermal cells to be reprogrammed into cells of common developmental origin – both originate from the ectoderm - without genetic modification and under defined culture conditions. Finally, our work has the potential to provide a novel source of abundant, readily accessible, autologous stem cells for treatment of neurodegenerative diseases, for which cell sourcing remains a severe impediment hampering cell therapy approaches.


Sarah Shafaat
Department of Materials Science & Engineering, Kroto Research Institute, University of Sheffield, United Kingdom
Title: DEMONSTRATION OF IMPROVED TISSUE INTEGRATION AND ANGIOGENESIS WITH AN ELASTIC, ESTRADIOL RELEASING POLYURETHANE MATERIAL DESIGNED FOR USE IN PELVIC FLOOR REPAIR

Biography: Sarah Shafaat is from Department of Materials Science & Engineering, Kroto Research Institute, University of Sheffield, United Kingdom

Abstract: Pelvic organ prolapse and stress urinary incontinenceaffect 40-50% of postmenopausal women worldwide. Polypropylene meshes have been extensively used for the surgical intervention of these disorders; however, these meshes can lead to severe complications in some patients. The need for synthetic materials more suited for use in the pelvic floor repair is widely accepted. This study aims to develop an electrospun 17-β-estradiol releasing polyurethane (PU) scaffold that not only provides the appropriate mechanical support to the prolapsed pelvic organs but can also stimulate new extracellular matrix (ECM) production and angiogenesis. Methods: PU scaffolds with and without 17-β-estradiol (25mg/g and 50mg/g) were prepared by blend electrospinning. Mechanical properties of scaffolds were assessed by uniaxial cyclic and non-cyclic testing. The viability and ECM production of human adipose derived mesenchymal stem cells (hADMSCs) cultured on 17-β-estradiol releasing PU scaffolds was evaluated. Angiogenic potential of estradiol releasing scaffolds was demonstrated by using an ex ovo chick chorioallantoic membrane (CAM) assay. Results: The inclusion of estradiol in PU scaffolds did not change the ultrastructure but it significantly increased the ultimate tensile strength of scaffolds. hADMSCs on estradiol-releasing PU scaffolds showed more ECM production. The CAM assay revealed a significantly higher angiogenic potential of estradiol-releasing PU scaffolds with an additive effect seen when hADMSCs cultured on estradiol scaffolds. Histological examination of CAM tissue sections showed extensive cellular infiltration and a good tissue integration for all constructed scaffolds. Conclusions: This study shows the angiogenic potential of estradiol-releasing PU scaffolds with appropriate strength and elasticity desirable to support the pelvic floor.


Mohammad Nazrul Islam
Head-Biomedical Science and Medical Biotechnology Department, ShaheedSuhrawardy Medical College and Hospital, Bangladesh
Title: SOFT TISSUE WOUND HEALING BY LASER

Biography: Dr.Mohammad Nazrul Islam has completed his MBBS degree from DhakaUniversity, and later M.Sc.(BME)from Gono-biswabidyalaya, Dhaka, Bangladesh. His professional intern/trainingwas done at Carnegie Hill Institute, New York,USA. He is the foundinding head of Biomedical and Medical Biotechnology Department of ShaheedSuhrawardy Medical College and Hospital, Dhaka. He has published papers in reputed professional, national/international forum/ journals and continues academic/ research work at ShaheedSuhrawardy Medical College and Hospital since 2007. Here is a brief description of his academic achievement and work placement over the past years.

Abstract: Background: In 1967 a few years after the first working laser was invented, EndreMester in Semmelweis University Budapest, Hungary wanted to find out if laser might cause cancer. He took some mice, shaved the hair off their backs, divided them into two groups and gave a laser treatment with a low powered ruby laser to one group. They did not get cancer and to his surprise the hair on the treated group grew back more quickly than the untreated group. That was how "laser biostimulation" effects were discovered. (Effect of laser on hair Growth of mice (in Hungarian).Mester, E. Szende, B. and Tota, J.G. (1967).KiserlOrvostud 19. 628-631). Purpose of the work: The effects of pulsed monochromatic light, with fixed pulsations and wavelengths, on the healing of pressure ulcers were evaluated in this prospective, randomized, controlled study. Method: A placebo-controlled, double-blind study using low energy photon therapy (LLLT) was performed in ten patients with bedsore on the back. Treatment was given three times a week for 10 weeks, using monochromatic (red) optical sources; diode 660nm (GaAl-660). The patients who were randomized to placebo treatment received sham therapy from an identical-appearing light source from the same delivery system. Results: Ten patients with bedsore were randomized to receive LLLT or placebo therapy. At the conclusion of the study, the percentage of the initial ulcer area remaining unhealed in the LLLT and placebo groups was 24.4% and 84.7%, respectively (P = 0.0008). The decrease in ulcer area (compared to baseline) observed in the LLLT and placebo groups was 193.0 mm2 and 14.7 mm2, respectively (P = 0.0002). One patient dropped out of the study, complaining of lack of treatment efficacy; he was found to be randomized to the placebo group. There were no adverse effects. Conclusions: In this placebo-controlled, double-blind study LLLT was an effective modality for the treatment of bedsore which were resistant to conventional medical management. The results are encouraging as pulsed monochromatic light increased healing rate and shortened healing time. This will positively affect the quality of life in elderly patients with pressure ulcers. Keywords: Bedsore Healing, Soft Tissue Healing, Decubitus Ulcer,Ulcer Healing, Wound Healing, LowLevel Laser, Laser Therapy.


Dr Abdul Hakeem
University of Leeds, LS2 9JT, UK.
Title: Production and Characterisation of Acellular Human Liver Matrix: Potential Auxiliary Liver Graft

Biography: Dr Abdul Hakeem is from School of Biomedical Sciences, Institute of Medical and Biological Engineering, University of Leeds, LS2 9JT

Abstract: Shortage of donor organs combined with ever increasing liver disease has directed attention towards growing implantable liver tissue in the laboratory setting. Recent developments in decellularisation and recellularisation techniques have opened exciting facets for liver whole organ reengineering. The aim of this study was to develop a decellularisation protocol for human liver, assess the structural and biochemical integrity and assess the biocompatibility of the acellular matrix. Discarded human livers were split and the left lateral lobes with a mean weight of 399 ± 162 g were used. Perfusion decellularisation was carried out through the hepatic artery and portal vein. Hypotonic buffer, 0.1 % (w/v) sodium dodecyl sulphate, hypertonic buffer and nuclease solutions were used to remove cells plus nuclear material from the liver tissue. Following several iterations of a previously patented Leeds University decellularisation protocol, greater than 91.0 % DNA was removed from all areas of liver tissue. Histological analysis demonstrated lack of cells and maintenance of the portal triad histioarchitecture. Immunohistochemistry demonstrated positive staining for key extracellular proteins such as collagen type I and III, fibronectin and laminin. Scanning electron microscopy showed an intact extracellular matrix. Biochemical assessment confirmed significant reduction in glycosaminoglycan content, but there was increase in collagen content, the latter likely due to extraction by dry weight of other soluble components in the matrix. The acellular tissues and extracts were not cytotoxic to either murine 3T3 or baby hamster kidney cells. Preliminary recellularisation work showed that the matrix is biocompatible to primary cryopreserved human hepatocytes seeded at 30,000 cells per m2 density. The study has developed a suitable protocol for decellularisation of the left lateral lobe of human liver, without adversely affecting the extracellular matrix structure. The biocompatible acellular scaffold has the potential to be recellularised with hepatocytes, with a view to engineering a transplantable auxiliary liver graft.


Biomaterials Engineering and Biodegradable Biomaterials

Session Introduction

Chien-Yu Lin
National Cheng Kung University Department of BioMedical Engineering, Laboratory of Nano Biomechanics , Taiwan.
Title: In-vivo study of a biodegradable magnesium anchor for suture anchor technique of rotator cuff repair

Biography: Chien-Yu Lin obtained herBachelor’s Degree from the department of BioMedical Engineering at National Cheng Kung University, Taiwan in 2017. My independent study mainly focused on the development of conversion coatings on magnesium alloys for orthopaedic applications. The interest in biodegradable implants prompts me to continue my research in this field. I started my Master study under Prof. Ming-Long Yeh’s guidance in the same department. Currently, we’re working with Dr. Wei-Ren Su and Dr. Fa-Chuan Kuan in Orthopaedic surgery at the National Cheng Kung University Hospital and Prof. Fei-Yi Hung in the department of Materials Science and Engineering at National Cheng Kung University. The main purpose of our research is to develop a biodegradable magnesium anchor for suture-anchor technique of rotator cuff repair

Abstract: Suture anchor technique is one of the most commonly used surgical treatment of rotator cuff tear. The high retearing rate of repaired rotator cuff by anchor made of titanium alloy due to consistent foreign body reaction or polymers due to inferior mechanical strength and osteolysis concern remains a challenge in arthroscopic rotator cuff repair. Biodegradable material is the new generation of biomaterials served as the tissue integration scaffold for high regenerated tissue. Therefore, examination of a degradable magnesium anchor is the aim of this study. Recently, biodegradable magnesium alloy has attracted great attention because it has perfect biocompatibility, osteointegration ability and close mechanical properties to natural bone. However, the corrosion behavior of these metals in-vivo remains challenging. In this study, a protective magnesium fluoride (MgF2) coating was deposited on the surface of Mg-Zn-Zr (ZK50) alloy anchor and the cell viability tests showed that MgF2-coating could stimulate osteoblastic cell proliferation. So, the MgF2-coated ZK50 anchors were used on rotator cuff repair of rabbits’ shoulders. Non-degradable titanium anchors were used as control group in this approach. 24 New Zealand white rabbits were randomly divided into 2 groups: Mg group and Ti group, and the release of the supraspinatus tendon was performed at the base of the tendon insertion area. Anchor was inserted into greater tuberosity, then the detached tendon was repaired by single-row suture method. Pullout tests were conducted at 3 days after surgery to compare the initial fixation provided by suture anchor for the torn tendon. Micro-CT scans and histological analysis were performed at 1, 4 and 12-week post-operation to observe the biocompatibility of the implants and the change of degradation products. Preliminary results showed that MgF2-coated ZK50 anchor can degrade at proper time during the tendon-bone healing and potentially offer a novel material for next-generation anchor application.


Rana Imani
Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran,
Title: Multi‐functionalized Graphene Oxide Nano-carrier, Potential for Localized Delivery of Lysosomotropic Agent, for

Biography: Rana Imani (born 1984) is assistant professor of tissue engineering group, biomedical engineering department, at Amirkabir University of Technology (Tehran polytechnic). She received her PhD (2015) in biomedical engineering (Biomaterials) from Amirkabir University of Technology. In the last year of her PhD, she participate in an exchange program at the University of McGill (Montreal, Canada) to research in the field of nano-carrier design for gene therapy applications. She has jointed to tissue engineering group of Amirkabir University of Technology from 2016. Her main research interests include gene and drug delivery systems, nano-carriers design, tissue engineering scaffolds, 3D cell bioprinting, graphene based nano-compoits and in-situ formed hydrogels for biomedical applications.

Abstract: Nowadays, gene therapy serves as a key platform technology, which has demonstrated increasing promise for treating a variety of genetic-based diseases. The success of nucleic acids-based therapy is largely dependent on the safe and efficient delivery system. Here, a muti-functionalized graphene oxide (GO)-based nanocarrier with conjugation of aminated-polyethylenglycole (PEG-diamine) and octaarginine (R8) for intra-cellular nucleic acids delivery is proposed. The two functionalities are covalently co-conjugated and the PEG: R8 ratio is optimized. The optimized nano-carrier was further functionalized with folic acid (FA) and a lysosomotropic agent (CQ) to add the targetability to cancer cells and enhanced transfection efficacy, respectively. The successful synthesis of the multi-functionalized nanocarrier was comprehensively studied using FITR, Uv-vis spectroscopy, TGA, SEM, TEM, DLS and zeta sizer. The potential of the nanocarrier to localized release of CQ inside the cell lysosomal compartment was studied. The ability of breast cancer cell lines including MCF-7 and MDA-MB 231 for uptake of optimized nano-carrier is investigated. Furthermore, the delivery of functional genes is assessed using c-Myc protein knocking down. The optimized nano-carrier formulation, which was obtained via the co-conjugation approach showed 0.068 μ mol/mg of FA with +31.97 mV and 224 nm charge and size, respectively, and remained stable in biological solution. FA introduction to R8 and PEG functionalized nano-carrier significantly enhanced the rate of MCF-7 cellular internalization via receptor- mediated endocytosis, which was confirmed by TEM observations. However, the macropinocytosis pathway was also induced as a result of R8 functionality. Incorporation of CQ at optimized concentration of 10 μM via π-π stacking empowered the nanocarrier to escape from the lysosomal compartment due to the lysosomotropic effect of CQ. Furthermore, the pH dependent release profile of CQ (95.3% in pH 4.5) from nano-carrier provided more efficiency and safety than free CQ treating. The MTS and western blot analyses demonstrated that the two functional siRNA, cell death control siRNA and anti c-Myc siRNA, delivered by the multi-functionalized nano-carrier were highly functional after successful transfection of the cells. As summary, it is highly believed that the synthesized and optimized multi-functional nano-carrier has great potential to be utilized as a siRNA nano-carrier for potential targeted cancer therapy applications. Keywords: Graphene oxide; Nano-carrier, Multi-functionalization; Cell penetrating peptide; Targeting; Lysosomal escape; siRNA.


Li-Fang Lin
National Cheng Kung University, Taiwan.
Title: Synthesis of benzoic acid-modified poly(L-lysine) star polypeptide as a potential anti-inflammatory agent in Lipopolysaccharide-induced sepsis

Biography: Li-Fang Lin, obtained BSc in technology and management from National Tsing Hua University, Taiwan in 2016. My senior thesis mainly focus on the effect on hydrophilic drug delivery by themosensitive mPEG-Alanine hydrogel. The interest in drug delivery system and polymer biomaterial promotes me to research on related fields. I started my master degree in department of biomedical engineering at National Cheng Kung University, Taiwan. The main research is functional biomaterials as anti-inflammatory agents and other biochemical analysis.

Abstract: Sepsis is a systemic inflammatory response caused by bacteria infection. It is known that star-shaped structure polypeptides efficiently interact with the outer membranes of bacteria and enhance antimicrobial activity. For this, linear and star polypeptides were synthesized by ring-opening polymerization (ROP) of N-carboxyanhydrides (NCAs)with benzoic acid modified as functional group. In this work, we found that star polypeptidewith modification inhibited the expression of nitric oxide and pro-inflammatory mediators such as TNF-α, IL-6 from the LPS-stimulated RAW264.7 macrophages and exhibited significant anti-inflammatory properties as well compared to linear modified polypeptide and non-modified star polypeptide. Mechanistic studies revealed that the inhibitory effects of modified star polypeptide was mainly mediated by the inhibition of phosphorylated JNK and the down-regulated nuclear translocation of p65 expression. Furthermore, modified star polypeptide suppressed TNF-α, IL-6 in the LPS-induced sepsis mice model. These results support that this simply synthesized star polypeptide with additional functionality is a promising anti-inflammatory agent for bacteria induced diseases.


Dr. W.J.P. Barnes
Institute of Molecular, Cell and Systems Biology, University of Glasgow
Title: Adhesion and friction in tree frogs and their relevance to the development of new smart adhesives

Biography: Educated at Leighton Park School in Reading, England, before coming up to Scotland to study at St Andrews University. There I obtained an Honours degree in Zoology and a PhD in invertebrate neuroscience, before moving to Glasgow University to an academic post. 50+ years later, I am still there, having spent 40 years in the Zoology Department, retiring as Reader, before moving to the Centre for Cell Engineering, where I am an Honorary Research Fellow. I began my research career as an invertebrate neuroscientist, but for the last 15 or so years have mainly studied adhesion and friction in tree frogs and their implications for biomimetics.

Abstract: The mechanisms of adhesion in climbing animals have many properties that are the envy of engineers, and therefore have enormous biomimetic potential. These include (a) good adhesion on many substrates (including wet ones), (b) reversible adhesion and re-usability, (c) self-cleaning and (d) only sticking when required. Gecko research has led the way in this field, but tree frogs have evolved a very different mechanism of adhesion, and are therefore worthy of investigation. Such research lies on the border between biology and materials science. In this talk, I will describe how a variety of approaches provide insights into why tree frogs adhere so well to overhanging surfaces (my lab in Glasgow), work on the fabrication of tree frog inspired adhesive surfaces (del Campo, Butt and Kappl, my collaborators at the Max-Planck-InstitutfürPolymerforschung, Mainz) and briefly describe some of the devices that have been inspired by these investigations.


Raniah S Alotibi
King Saud bin Abdul-Aziz University for Health Sciences, Riyadh, KSA
Title: The role of oxidative stress as a mutational mechanism on telomeric deletion events

Biography: Raniah Alotibi, Ph.D. is a Assistant Professor, Cancer and Genetics, College of Applied Medical Sciences, King Saud bin Abdulaziz University for Health Sciences, Saudi Arabia

Abstract: Telomeres are nucleoprotein structures that contain non-coding (TTAGGG) tandem repeats and associated telomere binding proteins at the end of chromosomes. As a consequence of end-replication losses, telomeres undergo gradual erosion with ongoing cell division. It is hypothesised that in addition to the end-replication problem, mutational mechanisms may contribute to telomere erosion and generate large-scale telomeric deletions. As short dysfunctional telomeres are capable of fusion to other chromosome ends, large-scale telomeric deletion can lead to genomic instability that may drive tumour progression. The aim of this study is to observe if oxidative stress contributes to telomere erosion and large-scale telomeric deletion. By undertaking a comprehensive analysis of telomere dynamics following the induction of oxidative stress, the data presented here showed that oxidative damage does not appear to affect the rate of telomere erosion or the frequency of large-scale telomeric deletion. Instead prolonged exposure to oxidative stress results in the preferential loss from the culture of sub-populations of cells that exhibit short telomeres. We conclude that loss of these cells from the culture may be due to a preferential sensitivity to damage that may be related to these cells being closer to their replicative limit. These data are more consistent with the view that premature senescence does not arise as a consequence of accelerated telomere erosion, but instead more likely results from stochastic DNA damage across the rest of the genome.


Je-Wei Liu
National Cheng Kung University, Taiwan.
Title: Enhancement of corrosion resistance of phytic acid conversion coating on bioresorbable magnesium alloys using PLGA for orthopedic use

Biography: Je-Wei Liu is currently a master student majoring in biomedical engineering at National Cheng Kung University in Taiwan. The topic of his undergraduate research was The effect of storeoperated 𝐶𝑎2+ entry on focal adhesion dynamics and cell migration. He have been working on the surface modification for bioresorbable magnesium-based materials during his graduate years. The topic of his current study is Enhancement of corrosion resistance of phytic acid conversion coating on bioresorbable magnesium alloys using PLGA for orthopedic use.

Abstract: In recent years, magnesium alloys have been considered as novel bioresorbable materials. Magnesium alloys possess good biocompatibility, lightweight property, mechanical properties similar to natural bones and bioresorbability, making them become new alternatives over conventional titanium alloys and polymeric materials as orthopedic implants materials. The drawback of magnesium alloys is the poor corrosion resistance leading to high degradation rate in human bodies. Hence, surface modification is needed to enhance their corrosion resistance. In this study, chemical conversion treatment with phytic acid (PA) was used to deposit a protective coating on high purity magnesium (HP-Mg). In order to seal the micro cracks appearing on the coating, PLGA was used to manufacture a PA/PLGA composite coating. The SEM photos and EDS analysis showed that PLGA successfully sealed the micro cracks on the PA conversion coating, giving it a smooth surface. The hydrogen evolution test and polarization curves confirmed that the corrosion resistance was further enhanced after cracks were sealed with PLGA. Cytotoxicity, cell adhesion and wound healing assay indicated that PA/PLGA composite coating was biocompatible and did not pose bad effects on bone cells. Therefore, from the results above, the PA/PLGA composite coating manufactured in this study is a suitable candidate for the protection of magnesium implant materials and is not toxic to human bodies.


Biomaterials for Therapeutic and Investigative Delivery

Session Introduction

Dr. Qiang Qingfen
Department of Otolaryngology, Head and Neck Surgery, Xiangya Hospital, Central South University, China
Title: A novel calcium phosphate based and polyethyleneiminecoated non-viral genevector for innereargenetherapy

Biography: Dr. Qiang Qingfen, MD, nowis a Ph.Dstudent of OtolaryngologyHead & Neck Surgery, XiangyaHospital, Central South University, and herPh.D supervisor is Dr. Sun Hong. Shegother MD at XiangyaHospital in 2014. Dr. Qiang Qingfenfocused on the researches of preperation of nanoparticlevector for innereargenetherapysince 2014. Nowsheisstillstudyingthis area and sparing no effort to do the research.

Abstract: The aim of thisstudywas to develop a calcium phosphate (CaP) basedvector for innereargenetherapy. Nanoparticleswith a core of size-definedCaPwascoated by ethyleneimine(PEI) whichserved as surfactant to providecolloidalstabilizationwith high positive charge, and combinedwith a recombinant plasmidcarrying EGFP (structure CaP/PEI/ DNA, fromcore to outside). This newlydevelopedvector system wascharacterized and tested on differentcelllines (293t, HeLa, 5-8f, 6-10b) assessing by expression of EGFP. Lipofectamine® 2000 wasused as positive control. To evaluateitspotential application in genetherapy, therapeuticgene XIAP (encoding X-linkedinhibitor of apoptosisprotein) combinedwith EGFP report genewasloaded for cell transfection in Helacells. Meanwhile, organotypic culture of the organ of Corti and primary culture of stria vascularis marginal cellfrom SD rats wereexposed to the novelvector. The in vivo transfection efficiency and cytotoxicityweretested. This nanoparticlegenevectorshowed high transfection efficiency in all the testedcelllineswith more than 50%, and muchlowercelltoxicitythan Lipo2000 by MTT assay. Furthermore, the transfection efficiencydid notdecreaseobviouslyafterloadingtherapeutic XIAP-EGFP gene in HeLacells. It wasalsoobserved to transfer to cells in the interiorregion of basilar membrane fromRosenthal’s canal and transfectprimary culture of stria vascularis marginal cellsuccessfully. This novelnanoparticlevector system withadequate transfection efficiency, biocompatibility and high carryingcapacity of loadingtherapeuticgenecouldpossibly serve as a practical non-viral genetherapyvector, and isworthy of more researches in the future to applythisvector in innerear in vitro and in vivo


Sabyasachi Mukhopadhyay,
Weizmann Institute of Science, Israel
Title: Biological Fingerprints in Molecular Electronics

Biography: Sabyasachi Mukhopadhyay obtained his M.Sc. in Physics from IIT (Kharagpur) and Ph.D. from Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore, India in organic photovoltaics. He was a PD fellow at Weizmann Institute of Science (2013-2016) and recipient of a PBC PD Fellowship for 2013.Presently, he is affiliatedto the Physics Department, SRM University AP, Amaravati, Indiaas an Assistant Professor (since June 2017). His research interests involve developing advanced instrumentation and electronic characterization techniques to understand electron transport processes across various molecules and thin films towards efficient energy conversion applications.

Abstract: Electron transfer in and across proteins plays a key role in many biological processes of organisms. Fundamental understanding electron flow within protein structures is not only important for biology, but may also help in the design ofbio-electronic devices that directly couple to biological processes. Electrochemistry of redox-active proteins and enzymes is widely used to study electron transfer processes in those processes, while the impact of external voltage-driven electronic transportacross bio-molecular junctions has been limited because of limited information that could be obtained from resulting current-voltage characteristics.However, as we found,protein-based molecular junctions coulddemonstratedistincttransport efficiencies, i.e.junction-currents, depending on protein orientation, conformation,mutation and especially, the presence of cofactors. Molecular junctions with photoactive membrane proteins and photochromic photoreceptor proteins, which alter their conformation upon light absorption, demonstrate modulation in junction-current with illumination. Removing or modifying protein cofactors not only shows direct effects on junction currents, but also a transition from tunneling (temperature-independent) to temperature-dependent transport. The high conducting nature of the proteinjunctions raises questions about the fundamental nature of electron transport across proteins. Finally, we will demonstratehow these biomolecular signatures from molecular electronic transport can beused to map electron transport paths within the protein.Detailed knowledge of nanoscale conduction pathways would enable developing synthetic proteins with higher conductanceand different functionalities, whichwill directlyaffectthe field of bioelectronics.


Raniah S Alotibi
College of Applied Medical Sciences,King Saud bin Abdulaziz University for Health Sciences, Saudi Arabia
Title: The role of oxidative stress as a mutational mechanism on telomeric deletion events

Biography: Raniah Alotibi, Ph.D. is a Assistant Professor, Cancer and Genetics from College of Applied Medical Sciences,King Saud bin Abdulaziz University for Health Sciences, Saudi Arabia

Abstract: Telomeres are nucleoprotein structures that contain non-coding (TTAGGG) tandem repeats and associated telomere binding proteins at the end of chromosomes. As a consequence of end-replication losses, telomeres undergo gradual erosion with ongoing cell division. It is hypothesised that in addition to the end-replication problem, mutational mechanisms may contribute to telomere erosion and generate large-scale telomeric deletions. As short dysfunctional telomeres are capable of fusion to other chromosome ends, large-scale telomeric deletion can lead to genomic instability that may drive tumour progression. The aim of this study is to observe if oxidative stress contributes to telomere erosion and large-scale telomeric deletion. By undertaking a comprehensive analysis of telomere dynamics following the induction of oxidative stress, the data presented here showed that oxidative damage does not appear to affect the rate of telomere erosion or the frequency of large-scale telomeric deletion. Instead prolonged exposure to oxidative stress results in the preferential loss from the culture of sub-populations of cells that exhibit short telomeres. We conclude that loss of these cells from the culture may be due to a preferential sensitivity to damage that may be related to these cells being closer to their replicative limit. These data are more consistent with the view that premature senescence does not arise as a consequence of accelerated telomere erosion, but instead more likely results from stochastic DNA damage across the rest of the genome.


Design of Novel Functional Biomaterials

Session Introduction

Liliana Rubio
Polymer Business Intelligence Innovation Project Management Office, Brazil.
Title: SKYN OF THE FUTURE Innovation & Sustainability in the contemporary Market

Biography: Liliana Rubio is a Chemical Engineer, Master in Project Management, MBA in Business Administration, MBA in Innovation Management, Specialist in polymer; focused trainings in Europe, USA and Latin America. Over 18 years’ experience working on new business, R&D and marketing in the polymer and Textile Industry. PMO Polymer Business Intelligence is a Project Management Office, located in Sao Paulo-Brazil, dedicated to project management consulting, mentoring, covering project planning,implementation and execution through a front-to-end creative quantification approach. Founder of SmartTex Hub ecosystem for the value chain of textile Industry and final applications. Winner of the Clariant Corporation Innovation Extra Award 2007: The sustainable project for the Green Industry on renewable resources. Winner Honorable Mention in Think Beyond Plastics 2016 innovation competition on the category Most Innovative Emerging Business as part of the team "Plastic Smog Emissions Closed Loopon" with Modern architecture project K-Rubio Smart House (biocomposites from waste micro plastic particles (beads and fibers). Speaker at conferences and lectures on sustainability and innovation at universities and the main trade fairs of polymer Industry in Latin America and EUA. Author of several articles that have been publish in technology and business magazines

Abstract: On the dynamic business scenario, Be Functional, Smart and Interactive are the most valuable asset to become a reference in our markets. The global Smart textile market promotes the implementation of Nanotech and Bio composites projects in this field. Please note the market by moving beyond traditional path of what means value and fulfilling business future. We can identify the strategic alliance between Textile industry and several markets as Polymer, Cosmetic, Health, Architecture and Fashion. For several years, while these "S-textile" program have been able to go beyond the original objectives and is seeking its way towards industrialization and mass production for enhancing the breakthrough of intelligence textile systems. Every Innovative initiative are committed in improving the convergence between industries and the leading edge of the Textile market; on this scenario is a priority the deep understanding of megatrends and new segments. We discuss about the most important trends that will define the architecture future of Smart Textil world