E-mail: secretary@worldbiocongress.com | USA : +1-646-828-7579, UK : +44-203-695-1242 | August 21-23, 2017, Barcelona, Spain  


Microbiology and Microbial World

Session Introduction

Gema Bárcenas Moreno
Professor, University of Seville, Spain
Title: Fire and soil microorganisms: where should we focus on?

Biography: Gema Bárcenas-Moreno studied Biology degree at Granada University. She developed her PhD studies about soil quality in Mediterranean area at Agrochemistry and Environment Department of Miguel Hernandez University where received the doctorate at 2010. She has realized three short stays at Lund University (Sweden) at the Microbial Ecology Department under supervision of Dr. Erland Bååth. Actually, Gema Bárcenas is member of MedSoil Research Group and is teaching at Seville University. During this new situation she has establish collaboration with the Department of Microbiology at the University of Granada, to deepen the fungal degrading capacities and their genetic identification.

Abstract: The study of wildfire effect on soil microorganisms and the implication of microbial response in ecosystem recovery has been one of the objectives of our research projects in the last decade. Forest fire in Mediterranean area can be consider as part of the ecosystem in itself, since it has conditioned the adaptation of vegetation from their origin and even some authors consider fire as the eighth factor of soil formation. Our first steps studying fire effect on soil microorganisms were inspired by the search of fire severity indicators that could be used by monitoring tool and give us the opportunity to establish ecosystem recovery information based on the fast response and sensitivity of soil microorganisms. Nevertheless, the results obtained showed us immediately the high variability and complexity of microbial world. The use of field studies combined with laboratory heating has given us the opportunity to find some crucial factors condition microbial recolonization after fire as preliminary soil pH and fire-induced pH changes, and which method or microbial parameter should be measure depending on the time elapsed since the fire. The collection of investigations related to each other has allowed us to focus the new projects towards a more applied use of the measurement of microbial parameters in the soil. We are currently looking for greater applicability of microbial response follow-up to assess post-fire management and to establish possible post-fire behaviors of certain environments in function of their vegetation and the associated microbial community. Our objective for this meeting is to summarize the results obtained during the last years, review the state-of-the-art and share the ideas which are around our mind in order to find new and fresh inspiration to continue the adventure in this expedition through the mysterious microbial world.

Biodiversity, Energy Systems and Environmental Sustainability

Session Introduction

Gholamhossein Hosseini
Assistant Professor, Cotton Research Institute of Iran, Iran
Title: Earliness and suppression of its additive genetic effects by environmental and management factors in cotton (Gossypium hirsutum L.).

Biography: Gholamhossein Hosseini pursued M.Sc. in Plant Breeding and later Ph.D in Biology Biometrical genetics. His research interests include Biometrical genetics, advanced statistics including PCA analysis, Factor analysis, Multiregression Genetics and Plants breeding of Cotton and Sunflower. Molecular genetics (SSR), Teaching of advanced statistics, quantitative genetics, genetics, heredity, cellular and molecular biology.

Abstract: Earliness is an important breeding objective in upland cotton improvement. An experimental project for integration of earliness genes from sindose-80 to bulgare-557 with assuming that inheritance pattern of earliness characteristic in cotton cultivars controlling by additive model of genetic effects carried out during 2005 to 2016 in the Department of Botany, University of Pune and Agricultural Research Center of Tehran-Iran. With this arrangement that for single cross between the two above mentioned lines in 2005 and 2011, five years selection have been done after every crosses. Obviously the second cross in 2011 considers as back cross. In 2016 the new earliness genotype by two different planting dates compared with the five native and commercial cotton varieties in RCBD design. The criteria for earliness were (1) Early flowering days on the basis of germination to first flowering (EFD). (2) Fraction of first picking to the total seed cotton yield (FFP). (3) Earliness Bartlett’s index (EBI) and finally (4) Earliness index of combined picking and day (CPD) which has been presented as a new earliness index in this paper. Comparison of the four mentioned formulae shows the effects of weight and time in the last accurate formulae. If the earliness criteria of cotton cultivars were compared in the inappropriate condition of cotton growth, the environmental and management suppression factors like soil moisture, soil fertility level, pest pressure, temperature and cloudy weather can affect earliness criteria which are controlling by additive quantitative genes despite of dominant qualitative genes.

Amer Jamil
Professor, University of Agriculture, Pakistan
Title: Evaluation of Molecular based Conservation Tool by Comparing rbcLa and ITS2 as DNA barcode regions

Biography: Prof. Amer Jamil is pioneer in the University of Agriculture Faisalabad Pakistan to develop and establish DNA barcoding facilities. He has completed several research projects including DNA barcoding of desert area and mountainous plants. He has contributed many research papers and review articles in reputed journals. He has presented his work in different conferences around the globe, and organized many international conferences and workshops. He has produced eighteen Ph.D. scholars, and many are working under his supervision. Prof. Jamil has also been awarded awards and fellowships.

Abstract: Reference DNA barcode resource for selected indigenous medicinal plants of Pakistan was generated in the current project that covered the most commonly used medicinal plants (130 species) belonged to different areas of Pakistan. The potential of DNA barcoding was tested on diverse taxa of medicinal plants by using core plastid gene region rbcLa and a nuclear region ITS2 as barcode regions. Vouchers and metadata of 258 specimens (triplicate of all species) were up loaded on BOLD with project name of "DNA Barcoding of Medicinal Plants of Pakistan (DBMPP)" after morphological identification. The success rates of the amplification of two DNA barcoding markers (rbcLa and ITS2) were 96% and 82% respectively using single primer pairs for each marker. Sequencing success rates were 100% (rbcLa) and 98% (ITS2). Gene region statistics were used as pre-screening test of DNA barcode sequences that revealed that ITS2 could be chosen as DNA barcode region for a dataset of diverse taxa. Mean pairwise distance, near neighbor analysis, best close match and BLASTn all methods of evaluation supported the hypothesis that ITS2 has the best potential to be used as barcode region for plants but have few limitations of amplification difficulty and alignment after sequencing. Combination of rbcLa+ITS2 was also tested that didn't improve the identification of species significantly. Method of BLASTn concluded that ITS2 assigned 99% samples (known to database) to species level while rbcLa assigned 78% to species level but it requires building comprehensive and authentic sequence libraries for sequence comparison beforehand. The same results were pictured in distance based Neighbor Joining tree. Total 621 sequences were generated in this study with 312 rbcLa and 309 ITS2. The practical utility of the region wise barcode sequence database is in the fields of health and medicine (herbal drug quality control), forensics, economy (to check illegal trade of threatened species) and conservation biology.

Bioinformatics and Biostatistics

Session Introduction

Sandra Milena Chingaté López
Research Scholar, Universidad Nacional de Colombia, Colombia
Title: Rational in silico design of analogue peptides derived of PMAP-36 and CAP-18 with improved antimycobacterial activity in vitro and inhibition of ATPase activity in plasma membrane vesicles of Mycobacterium smegmatis mc2155

Biography: Sandra Milena Chingaté López is a Chemist, Magister in Biochemistry Science and Doctoral Candidate in Biochemistry Science at Universidad Nacional de Colombia. She has developed her research in the area of antimicrobial peptides as new anti-TB compounds, focus on Cathelicidins and in silico design of peptides, in the research group Biochemistry and Molecular Biology of Mycobacterium at Universidad Nacional de Colombia.

Abstract: The emergence of multi drug-resistant Mycobacterium tuberculosis strains which are annually responsible for a large number of deaths worldwide, requires the development of new anti-tuberculosis agents. Membrane proteins are an interesting antimicrobial target, allowing the permeabilization of the cell membrane; for instance, the ATPase proteins have been studied as drug targets because they are involved in several cellular functions that allow cell viability, so the development of drugs as inhibitors of ATPase activity in pathogens is an interesting strategy. Antimicrobial peptides are molecules generated in the host innate response with effects on mycobacteria. PMAP-36 and CAP-18 are Cathelicidins from mammalians involved in the formation of channels in the cell membrane and bacterial lysis. In this study, the antimicrobial activity, cellular toxicity, as well as the effect on the mycobacterial cell membrane ATPase activity of PMAP-36 and CAP-18 derived peptides were assessed. By bioinformatic methods the rational design the amino acid sequence derived of PMAP-36 and CAP-18 with the best features of helical structure and antibacterial value were determined, the corresponding peptide called PAMPI-1 and CAPI-2, having the amino acid sequence: GRFRRLRKKTRKRLK and GRKIKLKLKKIGQKK, respectively. PAMPI-1 and CAPI-2 were synthesized by Fmoc strategy, purified by RP-HPLC and its structural characteristics were determined by mass spectrometry MALDI-TOF and circular dichroism. In vitro evaluation of PAMPI-1 and CAPI-2 were tested in strains of Mycobacterium smegmatis mc2155, obtaining a minimum inhibitory concentration of 37.5 μg/mL and 150 μg/mL, hemolytic activity of 4.77% and 4.04% and cytotoxic activity over murine macrophages of 725.9 μg/mL and 1359 μg/mL, respectivily. Interestingly, PAMPI-1 displayed approximately 26.9% inhibition of basal ATPase activity of the mycobacterial plasma membrane, which could in turn be associated with the impaired cell viability. The results suggest that PAMPI-1 could be considered as a potential antimycobacterial agent against target cell membrane ATPases.

Biochemistry, Cell and Molecular biology

Session Introduction

Tianhao Sun
Research Scholar, University of Hong Kong, Hong Kong
Title: miR-375-3p Negatively Regulates Osteogenesis by Targeting LRP5 and β-catenin

Biography: Tainhao Sun is a Research Scholar and he completed his Ph.D. from the University of Hong Kong. He has published many research papers and articles.

Abstract: Wnt signaling pathways are essential for bone formation. Previous studies showed that Wnt signaling pathways were regulated by miR-375. Thus, we aim to explore whether miR-375 could affect osteogenesis. In the present study, we investigated the roles of miR-375 and its downstream targets. Firstly, we revealed that miR-375-3p negatively modulated osteogenesis by suppressing positive regulators of osteogenesis and promoting negative regulators of osteogenesis. In addition, the results of TUNEL cell apoptosis assay showed that miR-375-3p induced MC3T3-E1 cell apoptosis. Secondly, miR-375-3p targeted low-density lipoprotein receptor-related protein 5 (LRP5), a co-receptor of the Wnt signaling pathways, and β-catenin as determined by luciferase activity assay, and it decreased the expression levels of LRP5 and β-catenin. Thirdly, the decline of protein levels of β-catenin was determined by immunocytochemistry and immunofluorescence. Finally, silence of LRP5 in osteoblast precursor cells resulted in diminished cell viability and cell proliferation as detected by WST-1-based colorimetric assay. Additionally, all the parameters including the relative bone volume from µCT measurement suggested that LRP5 knockout in mice resulted in a looser and worse-connected trabeculae. The mRNA levels of important negative modulators relating to osteogenesis increased after the functions of LRP5 were blocked in mice. Last but not least, the expression levels of LRP5 increased during the osteogenesis of MC3T3-E1, while the levels of β-catenin decreased in bone tissues from osteoporotic patients with vertebral compression fractures. In conclusion, we revealed miR-375-3p negatively regulated osteogenesis by targeting LRP5 and β-catenin. In addition, loss of functions of LRP5 damaged bone formation in vivo. Clinically, miR-375-3p and its targets might be used as diagnostic biomarkers for osteoporosis and might be also as novel therapeutic agents in osteoporosis treatment. The relevant products of miR-375-3p might be developed into molecular drugs in the future. These molecules could be used in translational medicine.

Dr. Niranjan Koirala
Chair, Department of Medical Biochemistry, Pokhara University, Nepal
Title: Sakuranetin, Genkwanin and Rhamnetin : Methylation , G lycosylation and In Vitro Anti - cancer Assays

Biography: Dr. Niranjan Koirala received his Ph.D. in Biochemistry-Pharmaceutical Engineering (2016) from Sun Moon University, Republic of Korea. He is an expertise in the field of Microbial-Industrial- Pharmaceutical Biotechnology, natural products chemistry, system engineering and synthetic biology including but not limited to genome mining, gene isolation using PCR, cloning and protein purification, enzyme characterization, gene deletion, metabolic engineering, metabolic co-factor channeling, strain development and engineering, synthesis using enzyme biocatalysis, production optimization, bioprocess-fermentation technology, phytochemicals, herbal and natural products isolation, modifications and testing their biological activities, HPLC, ESI-MS, GC-MS and NMR analysis. He has more than a dozen of international publications at his disposal and he is exceptionally a flair writer and researcher. He has been invited and participated in many national and international conferences. He is also serving in the editorial and reviewer board in several international journals. He has been awarded twice with the best poster presentation award and best oral presentation award in the international conferences; Dean’s Choice award awarded by Sun Moon University, South Korea; BRICPL Young Scientist award awarded by Biologics Research and Innovation Center, India; Shreepati Koirala Memorial Academic award, Certificate of appreciation by Koirala Welfare Society-Kaski, Nepal and Nepal Bidhya Bhushan “A” medal from the honorable President of Nepal to name a few selected ones. He is the lifetime member of Biotechnology Society of Nepal. Along with research, Dr. Koirala has experiences in supervising and mentoring undergraduate and graduate students. Currently he is serving as the Head of the medical biochemistry department at Nobel College, Pokhara University affiliate, Kathmandu, Nepal and he is also the Founder President and Research Director at Dr. Koirala Research Institute for Biotechnology and Biodiversity (Dr. KRIBB), an NGO, located at Kathmandu, Nepal.

Abstract: Escherichia coli harboring SaOMT2-MetK was employed for regiospecific7-O-methylation of naringenin, apigenin and quercetin. The maximum yield of sakuranetin, genkwanin and rhamnetin under optimized conditions was 194 μM, 170 μM and 196 μM respectively when 200 μM of naringenin, apigenin and quercetin were supplemented in the separate cultures. Further in this study, purified rhamnetin was used for the in vivo rhamnosylation in engineered E. coli IBR. Similarly, sakuranetin was also purified in large scale and used as a substrate for in vitro glycosylation by YjiC to produce glucose and galactose derivatives of sakuranetin. We also demonstrated greater pharmaceutical potential of sakuranetin, genkwanin and rhamnetin as anti-carcinogenic, anti-melanogenic and anti- angiogenic agents, compared to their unmethylated forms. Taken together this research work best describes the microbial production by host engineering, methylation for improvement of biological activities, glycosylation for enhancement of solubility and in vitro anti-cancer activities of the flavonoids being tested. This research work will open up the way for the use of sakuranetin, genkwanin and rhamnetin as anti cancer agents in near future.

Dr. Satyndra Kumar Yadav
Head and Director, Yathartha Research Institute, India
Title: Comparison of the neuroprotective potential of estrogen and levodopa in 1-methyl-4-pheny-l-1, 2, 3, 6-tetrahydropyridine (MPTP)-induced cognitive deficit in Parkinsonian mice model


Abstract: Parkinson’s disease (PD) is one of the most common neurodegenerative disease found in the aging population. Currently, many studies are being conducted to find a suitable and effective cure for PD, with an emphasis on the use of herbal plants. In recently, estrogen, a female sex hormone, is used as an anti-oxidant property. In this study, the neuroprotective effect of an estrogen is evaluated in the 1-methyl-4-phe-nyl-1,2,3,6-tetrahydropyridine (MPTP) model of PD with cognitive deficit and compared to Levodopa (LD), a well reported neuroprotective agent used for treating PD. Twenty-eight Swiss albino mice were randomly divided into four groups: Control, MPTP, MPTP+LD and MPTP + estrogen. The behavioral recovery in both LD and estrogen treated mice was investigated using the rota rod, foot printing, narrow beam walking test and hanging tests and non-motor behavioural recovery in both LD and estrogen treated was investigated using the Y-maize and morris water maize. Furthermore, we performed the biochemical test i.e. catalase, lipid and nitrite in prefrontal cortex as well as nigrostriatal region of mice brain. We also performed the acetylcholine esterase activity in prefrontal cortex and nigrostriatal region of mice brain. The recovery of dopamine neurons in the substantia nigra (SN) region was estimated by tyrosine hydroxylase (TH), immunostaining. Estrogen treatment restored all the deficits induced by MPTP more effectively than levodopa. Estrogen treatment recovered the number of TH-positive cells in both the SN region. Treatment with Estrogen significantly increased the levels of catalase, decreased the level of lipid and nitite in both region SN as well as prefrontal cortex region. Notably, the effect of estrogen was greater than that elicited by levodopa. Acetylcholine esterase activity was significantly increased in MPTP and it was decreased by the treatment of estrogen as well as levodopa but there was much greater significantly decreased in estrogen treatment. Our result suggested that estrogen treatment significantly reduced the MPTP induced neurotoxicity as evident by decrease in oxidative damage, physiological abnormalities and immunohistochemical changes in the Parkinsonian mouse with cognitive deficit as compared to levodopa treatment.

Sustainable Energies and their Development

Session Introduction

Hassan Azaizeh
Assistant Professor, Tel Hai college, Department of Environment, Israel
Title: Production of ethanol from olive mill solid wastes

Biography: Prof. Hassan Azaizeh has completed his doctoral degree from the University of Texas A&M, USA, B.Sc. and M.Sc. from the Hebrew University, Israel. He is a senior researcher at the Institute of Applied Research, The Galilee Society, Israel and Lecturer of Biotechnology and Environmental Sciences at the Tel Hai College. Prof. Azaizeh working on the production of bio ethanol from agricultural lignocellulose biomass in addition to the production of bio gas from agricultural organic waste such as olive mill wastewater. Working on the cultivation of different species of algae for the production of omega 3, beta-carotene and biodiesel.

Abstract: In the last decade there is much interest in bio-based fuels, especially as transportation fuel replacements, such as ethanol. Bio ethanol is considered as one of the most promising next generation fuels, as it is carbon neutral and can be produced from renewable resources like lignocellulosic material. To-date, most ethanol is produced mainly from grown biomass, raising issues of competition with food crops on land use, water and nutrients. One solution is the utilization of agricultural lignocellulose waste as a feedstock. Agriculture waste is rich in cellulose, hemi-cellulose and lignin. The process of converting lignocellulose to fuel ethanol requires: delignification to liberate cellulose and hemicellulose; depolymerization of carbohydrate polymers to produce free sugars; and fermentation of mixed hexose and pentose sugars to produce ethanol. One interesting such a waste is Olive Mill Solid Waste (OMSW). Olives are an important agro-industrial sector, where more than 6 million tons/year of OMSW are produced worldwide, mainly in the Mediterranean region. This waste is rich in cellulose and polyphenols and as of today it is considered an environmental nuisance without proper economical solution. We are looking into converting the cellulolytic fraction into ethanol, but the presences of lignin make the enzymatic hydrolysis a complex process, bringing up the need for effective pretreatment. Our objective is to test three pretreatment processes for OMSW: mild heat and pressure combined with weak acids; microwave with weak acids; and ozonation alone to find the best technique to expose cellulose to enzymatic hydrolysis. Our results showed that the microwave pretreatment converted ca 100% of cellulose to glucose at enzymatic hydrolysis. This pretreatment has an advantage over the pressure pretreatment that showed only 41% conversion. Even though the pretreatment using microwave or ozonation showed similar results in conversion of cellulose to sugars, but it has resulted in remarkably difference by the fermentation process. The fermentation of microwave treated OMSW resulted in ca 85% yield of ethanol in comparison to the ozonation which resulted only in 37%. The obtained results could facilitate the conversion of lignocellulosic of OMSW and other different biomass resources into ethanol.

Modern Biotechnology

Session Introduction

Dr Tebiesebeke
Research Scholar, Academy des Sciences et d ’Innovation, Wageningen University, Switzerland
Title: Constructible properties and combined bacterial species in pediatric nutrition

Biography: Dr. R. Te Biesebeke obtained his Ph.D. from the Top Institute of Food and Nutrition, the Wageningen University and Research Centre in the Netherlands, and a M.Sc. in Applied Molecular Biology from the University of Utrecht, and holds a Biochemistry Engineering degree from Saxion Polytechnics, the Netherlands. He started his career in Biochemical research in the Laboratoire de Biochimie of the Ecole Polytechnique l’X, Paris (France) and progressed his career in the Top Institute Food and Nutrition in biotechnology, nutrition and food technology (the Netherlands). He evolved his career in global operating organizations of excellence in R&D Nutrition, Food & Bioprocessing. Dr. R. Te Biesebeke is a Lead Scientist at Innovation Works (Switzerland), external expert of the World Health organization (Genève, Switzerland) and Head of Nutrition & Metabolism at l’Academie Internationale des Sciences et d'Innovation (Switzerland). He is dedicated to improve the quality of life of the general public in developed and developing countries, and more specifically, of infants, children and mothers of child bearing age.

Abstract: Probiotics are defined as live microorganisms that, when administered in adequate amounts, confer a health benefit on the host. Recent research has suggested that probiotics exert a wide range of effects through different mechanisms and sites of action, dependent on the host. Although the European Food Safety Agency has evaluated current probiotic strains insufficient characterized and clinical research ample for health claim approval, consumers (e.g. in the US) are interested in potential therapeutic and preventive health benefits. The Food and Agriculture Organization of the United Nations and the World Health Organization provide guidelines for probiotics: 1. Proper identification to the level of strain of all probiotics in the product, with deposit of all strains in an international culture collection 2. Characterization of each strain for traits important to its safety and function 3. Validation of health benefits in human studies, including identification of the quantity of the microorganism required to provide the benefit 4. Truthful and not misleading labeling of efficacy claims and content through the end of shelf life Over the last years we have seen examples of genetically modified strains with adapted physiological properties compared to the parenteral strain, as well as combinations of strains applied and these provide a start for prosperous future developments. The genetic modifications can impact improved survival/retention in the gastro -intestinal tract, cell cycle, cell wall, antibiotic resistance, and biochemical/metabolic properties of the strains. An excellent example is Lactobacillus reuteri which has been used widely in infant follow up nutritional formulas The current guidelines that any strain of microorganism that would be assigned to a group would be freed from the need for further safety assessment is insufficient to guarantee any health impact in short and long term. It is therefore crucial that improved guidelines allow flexible developments to secure quality and safety, specifically when it concerns infants and premature infants, or metabolically compromised individuals and when it concerns the possible long-term effects.


Session Introduction

Nazma Kiran
Army Medical College, Pakistan
Title: Morphometrical Analysis of Intervillous Space and Villous Membrane Thickness in Maternal Anemia

Biography: I have completed my post-graduation in Histopathology from National University of Sciences and Technology, Islamabad. Currently working as an Assistant Professor in the Department of Pathology, Rashid Latif Medical College, Lahore, Pakistan. I am author of a book titled “Placental Morphology at Different Maternal Hemoglobin Levels: A Histopathological study”, published in 2016, ISBN-13:978-3-639-86373-4.

Abstract: Background: Anemia in pregnancy is linked with an increased risk of preterm delivery, low birth weight, perinatal and maternal mortality and it is related with variable histo-morphological changes in placenta which show a reflection for the poor fetal outcome. The objective of this study is to assess the micro-morphometry of intervillous space and villous membrane of placenta in anemic mothers. Methods: This case control study was completed in one year. A total of 75 placentas were included, that were divided into study and control group. In control group (n=15) placentas were taken from mothers having normal haemoglobin levels and study group (n=60) included placentas from anaemic mothers having haemoglobin less than 11g/dl. Study group was subdivided into three groups, i.e., mildly (10.0-10.9g/dl), moderately (7.0-9.9g/dl) and severely (<7g/dl) anaemic group. Three representative sections were taken from placenta, i.e., one close to umbilical cord (A), one from periphery (C) and one midway between A and C (B).Ocular micro-meter was used to measure intervillous space and villous membrane thickness. Results: Intervillous space was prominent in study groups (41.26±16.33ìm) as compared to control group (15.98±3.81ìm) (p<0.05). Thickness of villous membrane was significantly less in study group as compared to control group (2.97±0.70) (p<0.05). Conclusion: The present study showed wide intervillous space and thin villous membrane in study group as compared to control group reflecting adaptive changes in response to hypoxia in maternal anaemia.

Biotechnology in Health Care

Session Introduction

Zhen Huan Liu
Professor, Guangzhou University of Chinese Medicine, China
Title: A Randomized Controlled Study and Evaluation of Children with Cerebral Palsy by Mind Acupuncture

Biography: Liu has worked on Clinical Integrative Rehabilitation of Child Neurology for 35 years, of which he has focused on the research of child rehabilitation of cerebral palsy and mental retardation for 29 years. He has treated exceeding 30,000 children from more than 20 countries including China, France, the USA, Japan and the UK, etc, and the effects of his treatments are in international advance. He has published more than 250 papers and 16 books. Since 1993, he has given special academic lectures on CP rehabilitation via Chinese Medicine to exceeding 20 countries, including the USA, the UK, Spanish, Norway, Netherlands, Brazil, France, Singapore, etc. He has won the second prizes of Provincial Prize for Progress in Science and Technology for his researches. In 1994, he won the Special Allowance of the State Council as a young-or- middle-aged Pediatrics expert with outstanding contribution and was adapted into the List of World-Famous Celebrities by the University of Cambridge of the UK. Till now, he has cultivated 25 PhD and M.Phil, as well as provided more than 150 doctors and therapists for around 30 provinces of China.

Abstract: To investigate the effects of clearing the Governor Vessel and refreshing the mind needling in neural development and remediation of children with cerebral palsy. Methods: 200 cases of children with cerebral palsy were randomly divided into the treatment group (n = 100) and the control group (n = 100). The treatment group was given the combined therapy of acupuncture and rehabilitation training for 3 months, while the control group was only treated with rehabilitation training. The Gross Motor Function Measure (GMFM) and Beijing Gesell Developmental Scale were adopted to assess the neural development and rehabilitation outcomes of the two groups. Skull CT/MRI was adopted to evaluate the plerosis of injured cerebral nerve after treatment. Results: The total effective rate in treatment group was 87%, significantly higher than the 55% in the control group. Conclusions: Clearing the Governor Vessel and refreshing the mind Needling could accelerate the recovery of injured brain nerve and the reconstruction of brain function and ameliorate both the motor development and cognitive development. The forward curative effect of acupuncture combined with rehabilitation training was significantly better than the pure rehabilitation training.

Zhen Huan Liu
Professor, Guangzhou University of Chinese Medicine, China
Title: The Clinical and Experimental Study on JianPiYiShen and TongDuXingNao Acupuncture for Children with Cerebral Palsy

Biography: Liu has worked on Clinical Integrative Rehabilitation of Child Neurology for 35 years, of which he has focused on the research of child rehabilitation of cerebral palsy and mental retardation for 29 years. He has treated exceeding 30,000 children from more than 20 countries including China, France, the USA, Japan and the UK, etc, and the effects of his treatments are in international advance. He has published more than 250 papers and 16 books. Since 1993, he has given special academic lectures on CP rehabilitation via Chinese Medicine to exceeding 20 countries, including the USA, the UK, Spanish, Norway, Netherlands, Brazil, France, Singapore, etc. He has won the second prizes of Provincial Prize for Progress in Science and Technology for his researches. In 1994, he won the Special Allowance of the State Council as a young-or- middle-aged Pediatrics expert with outstanding contribution and was adapted into the List of World-Famous Celebrities by the University of Cambridge of the UK. Till now, he has cultivated 25 PhD and M.Phil, as well as provided more than 150 doctors and therapists for around 30 provinces of China.

Abstract: In this study,146 cases of brain injury and 1078 cases of cerebral palsy were included by randomized controlled study with ICF (GMFM, Peabody fine motor function, Gesell, muscle tension, joint activity, ADL, TCD, skull B ultrasound, head CT / MRI, SPECT, DTI)evaluation method.The results showed that (1) The recovery rate of extracellular space (92.3%) was significantly higher than that of the control group (70.8%) (P <0.05) ,TCD total efficiency (79.3%) was significantly higher than that in the control group (51.8%) (P <0.05).JianPiYiShen and TongDuXingNaoacupunctureto promoting the development of neurological and cognitive movementunder 6 monthschildren,effectively reduce the neurological sequelae.(2) The total effective rate of the children with cerebral palsy was 87%in the acupuncture group, which was significantly higher than that of the control group (P <0.01).The total effective rate of CT / MRI was 59.55% in the acupuncture group and 13.25% higher than that in the control group (P <0.01). The total effective rate was 91.3% in the 1 year follow-up group, which was significantly higher than that in the control group (P <0.01). the FA value of white matter fiber bundle was significantly higher than that of acupuncture at 60 times (P <0.05).The recovery rate of ultrasonous brain injury (86.7%) in acupuncture group was significantly higher than that in control group (64.4%) (P <0.05). The recovery rate of SPECT in acupuncture group was 96.4%, which was significantly higher than that in the control group (P <0.01).(3) Acupuncture rehabilitation not only promote the development of white matter and gray matter in children with cerebral palsy, but also promote the brain function of children with cerebral palsy remodeling and compensation, and promote social adaptation, language and other cognitive function development, children with cerebral palsy movement and Fine motor function development and recovery, improve the children's self-care ability. The research is novel, the design is reasonable, the data is complete, the statistics are correct and the conclusion is credible. Has published 28 papers, including SCI included 3, published monograph 2, invention patents 3, the technology has been incorporated into the Chinese cerebral palsy rehabilitation guidelines (2015). Has a high clinical value and promote the use of value. The evaluation of acupuncture and moxibustion therapy and prevention of cerebral palsy children was not evaluated by ICF comprehensive evaluation system at home and abroad. Project leader after more than 30 years of clinical exploration and research, improve the cerebral palsy acupuncture massage rehabilitation technology, nearly 7 years in the Guangzhou University of Traditional Chinese Medicine Affiliated Nanhai Obstetrics and Gynecology Children's Hospital treatment of children with cerebral palsy 2236 cases achieved good results, and extended to Germany , The United States, Australia, the United Arab Emirates, Russia, Indonesia, Sri Lanka, Malaysia, Hong Kong, Macao, Taiwan and more than 30 provinces and cities nationwide, a total of 9408 cases of cerebral palsy treatment, the average effective rate of 85.25%, access to significant social benefits. The acupuncture rehabilitation technology to lead and promote the international pediatric cerebral palsy rehabilitation medicine development, reached the international leading level.

ICBB-2017| by: Scientific Future Group