E-mail: secretary@worldbiocongress.com | USA : +1-646-828-7579, UK : +44-203-695-1242 | August 21-23, 2017, Barcelona, Spain  

POSTER PRESENTATIONS

Zuzana Ježková
University of Chemistry and Technology, Czech Republic
Title: Optimization of cultivation of Salinispora tropica – attractive producer of new carotenoids

Biography: Zuzana Ježková pursued her Bachelor's degree in Biochemistry from Charles University. Later she pursued her Masters degree in Analytical Chemistry from the Same University. She completed her doctoral thesis on the topic "Biologically active substances from marine microorganisms". Her research interest includes bio-active compounds from marine organisms, biotechnology.

Abstract: Microbial diversity in seas and oceans constitute a large and valuable source of microorganisms for modern biotechnology. Marine organisms have a great potential for obtaining biologically active substances with a wide spectrum of applications. Interesting genus, which has not yet been completelyexplored, is for instance Salinispora tropica. It is a gram-positive bacteria classified by NCBI to order Actinomycetales [1].In both vegetative stage (orange colonies) and sporulation stage (black spores) it produces several secondary metabolites.This work was focused on exploiting the vegetative stage,in which it produces arecently isolated carotenoid called sioxanthin [2].The main objective was to optimize the cultivation of the bacteria under laboratory conditions.Cultivations were carried out in shake flask and bioreactor cultures.The following parameters were optimized: carbon (glucose, starch, glycerol) and nitrogen(inorganic salts, complex organic) sources, influence of light intensity and cultivation temperature.In addition, saline waste water obtained by demineralization of whey, was tested as an alternative source of minerals. The medium optimization was evaluated based on biomassand carotenoidproductivity. It was found that the alternative culture medium using saline waste wateris suitable for the cultivation of Salinispora tropica and can significantly decrease the production cost of microbial biomass. Simultaneously there were developed extraction and quantification methods of carotenoids. The antioxidant effect of carotenoids was tested on human cell lines (HEK, HeLa, HepG2, LNCaP and MCF7, Kyse30).


Young Min Lee
Konkuk University, Korea (South)
Title: Preventive and therapeutic effects of blueberry (Vacciniumcorymbosum) extract against DSS-induced ulcerative colitis by regulation of antioxidant and inflammatory mediators

Biography: Young Min Lee pursued his master’s degree at the department of life sciences in the Konkuk University, Global campus, South Korea. Interests also include food and medical research. He is recently pursuing the doctoral course with a degree in applied life science.

Abstract: Inflammatory bowel disease (IBD) is an inflammatory disorder caused by hyperactivation of effector immune cells that produce high levels of proinflammatory cytokines. The aims of our study were to determine whether orally administered blueberry extract (BE) could attenuate or prevent the development of experimental colitis in mice and to elucidate the mechanism of action. Female Balb/C mice (n=7) were randomized into groups differing in treatment conditions (prevention and treatment) and dose of BE (50 mg/kg body weight). Acute ulcerative colitis was induced by oral administration of 3% dextran sodium sulfate for 7 days in drinking water. Colonic mucosal injury was assessed by clinical, macroscopic, biochemical and histo pathological examinations. BE significantly decreased disease activity index and improved the macroscopic and histological score of colons when compared to the colitis group (Pb.05). BE markedly attenuated myelo peroxidase accumulation (colitis group 54.97±2.78 nmol/mg, treatment group 30.78±1.33 nmol/mg) andmalondialdehyde in colon and prostaglandin E2 level in serum while increasing the levels of superoxide dismutase and catalase (colitis group 11.94±1.16 U/ml,BE treatment group 16.49±0.39 U/ml) compared with the colitis group (Pb.05). mRNA levels of the cyclo oxygenase (COX)-2, interferon-γ, interleukin (IL)-1β and inducible nitric oxide synthase cytokines were determined by reverse transcriptase polymerase chain reaction. Immunohistochemical analysis showed that BE attenuates the expression of COX-2 and IL-1βin colonic tissue. Moreover, BE reduced the nuclear translocation of nuclear transcription factor kappa B (NF-κB)by immuno fluorescence analysis. Thus, the anti-inflammatory effect of BE at colorectal sites is a result of a number of mechanisms: antioxidation, downregulation of the expression of inflammatory mediators and inhibition of the nuclear translocation of NF-κB.


Aldo Sena de Oliveira
Professor, Federal University of Santa Catarina, Brazil
Title: Sulfonamides derived from carvacrol: a new class of potent anti-bacterial agents against resistant Staphylococcus aureus strains

Biography: Prof. Aldo Sena de Oliveira pursued his Bachelors degree in Chemistry from the Federal University of Lavras, Masters degree in Chemistry from the Federal University of Espirito Santo. Later, he pursued his Ph. D in Chemistry from Federal University of Santa Catarina, Brazil. He is currently an Adjunct Professor at the Federal University of Santa Catarina

Abstract: Bacterial antibiotic resistance is an increasing reality in modern medicine and it is emerging as a significant threat to public health [1] [2]. In general, bacteria may have intrinsic or innate resistance to drugs or the ability to acquire said resistance [3]. Moreover, the accelerated evolution and spread of antibiotic resistant bacteria has been impelled by the widespread overuse of antibiotics within human medicine and outside of human medicine in agriculture and aquaculture [4]. The drug resistance spectrum of S. aureus includes the principal classes of antibiotics in use or that have been used as penicillin, methicillin, fluoroquinolones and vancomycin [5]. Organisms that are resistant to all approved antibiotics involve treatment with experimental and potentially toxic drugs [6]. Consequently, it is necessary to discover new and more effective antibacterial agents to treat infections caused by antibiotic resistant bacteria. Sulfonamide moiety, -SO2NH-, occurs in a significant number of bioactive compounds with several activities including antibacterial [7] [8], antitumor [9], anti-carbon anhydrase [10], antidiabetic [11], hypoglycemic [12], diuretic [13], antithyroid [14], protease inhibitory activity [15] [16] and analgesic [17] among others. Sulfonamides were the pioneers among the chemotherapeutic agents systematically used for the prevention and cure against various diseases [18]. The low cost and low toxicity associated with this class of molecules makes them valuable for application in the synthesis of derivatives [19]. New sulfonamides derived from carvacrol were prepared through a reaction between 4-hydroxy- 2-isopropyl- 5-methyl benzene sulfonyl chloride with various amines in excellent yields (76- 98%). The sulfonamides were characterized using spectrometric and spectroscopic methods. Among these compounds, three derivatives showed excellent results in antibacterial activity against resistant S. aureus strains, with MIC values ranging from 3.9 to 62.50 ppm. The sulfonamide derivative of 4-methylaniline (SULF-1) had the best performance for all tested strains of bacteria (MIC = 3.9 to 15.62 ppm). Furthermore, the sulfonamide derivative of 4-fluoro aniline (SULF-3), which also presented promising results, was found to have a synergistic effect when combined with tetracycline and partial synergistic effect when combined with ampicillin, exhibiting an FIC index between 0.50 and 0.75. The sulfonamide derivative of 4-methylaniline had a synergistic effect in combination with erythromycin exhibiting an FIC index of 0.37. This encourages new tests to verify the applicability of these compounds in the therapy of infectious processes of difficult treatment today.


Md. Aminul Islam Apu
Islamic University, Bangladesh
Title: Zika virus: a threat for future

Biography: Md. Aminul Islam Apu had recently completed his Bachelor's degree in Biotechnology and Genetic Engineering, at Islamic University, Bangladesh. He published his research papers in several International Journals.

Abstract: Zika virus was from on several occasions from a mosquitoes named as Aedes africanus after its discovery in 1947 in Uganda. Zika virus (ZIKV) is an arbovirus belonging to the Flavivirus genus which was first isolated from a rhesus monkey in a forest of Uganda. Zika virus is the virus which is highly related with some virus like the West Nile viruses. Yellow fever, Japanese encephalitis, and dengue Zika virus as it continues its spread throughout the several islands of the West Pacific, non-endemic countries as Japan, Germany, Canada, Australia and United States and in many tropical regions around the world. Zika virus disease, in most causes no or only mild symptoms. While there is no specific treatment for the disease. Sometimes paracetamol can be effective for the disease. It is one of the major concern that this disease cannot be prevented by any of medications or any of vaccine. Recently the most of the people suffer from a specific problem due to this virus is this disease can spread the pregnant woman’s fetus which gradually causes several birth defects and other brain malformations.This review describes simply about the current understanding the epidemiology,clinical characteristics, transmission, and the diagnosis of Zika virus infection, as well as the future prospects regarding this Zika virus.


Siuming CHAN
Professor, Guangdong Ocean University, China
Title: Development of a biotechnique to manipulate growth in shrimp from the molecular molecular characterization of the Molt inhibiting hormone (MIH1) gene in the shrimp (Fenneropenaeus merguiensis)

Biography:

Abstract: The Molt Inhibiting Hormone gene and cDNA of the banana shrimp Fenneropenaeus merguiensis (FmMIH1) has been cloned and characterized. FmMIH1 possesses most of the characteristics of the eyestalk type-II CHH/MIH/GIH family neuropeptide. The open reading frame of FmMIH1 consists of 315 bp encoding for a protein of 105 amino acid residues. The mature peptide of FmMIH1 consists of 76 amino acid residues, a glycine residue at position 11 of the mature peptide and 6 cysteine residues located in the conserved position of the mature peptide. In addition to eyestalk, high levels of FmMIH1 transcript could also be detected in the intestine. FmMIH1 transcript level is low throughout the post-molt, early to mid-intermolt and premolt. However, a sharp increase could be observed in late intermolt (C3 stage). Both alignment and phylogenetic analysis reveal that FmMIH1 is most similar to the MIH1 of other shrimp species. For functional assay, RNA interference results show that a significant 2.3 days (P<0.05) reduction in molt cycle duration could be observed in shrimp receiving dsFmMIH1 injection. Surprisingly, injection of recombinant FmMIH1 could also cause a significant reduction of the molt cycle duration (average 1.9 days, P<0.05). In conclusion, the result of this study will provide us new insight to develop a technique to manipulate growth of shrimp in aquaculture.


Md Shariful Islam
Mawlana Bhashani Science and Technology University, Bangladesh
Title: Development of Treatment strategies to combat EHF (Ebola Hemorrhagic Fever): Possible sites of actions and targets for anti-Ebola drug discovery

Biography: Md. Shariful Islam is a young research student in the Faculty of Life Science and working in the Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Bangladesh. Md. Shariful Islam has published many articles, one of the articles is entitled as “Potential Drug Targets in the Death Pathway: Therapeutic Approaches in Apoptosis”. His research interests include Cancer Biology, Neuroscience, Biochemistry, Applied biochemistry, Physiology, Cellular Biochemistry, and Biotechnology. Recently he attended the 75th Annual Meeting of the Japanese Cancer Association as an oral speaker with travel and accommodation grants in University of Tokyo, Pacifico Yokohama, Japan. Recently Mr. Islam published an editorial on neuro biology entitled in “The Biochemistry of Hunger Stimulating Hormone: Why Understanding This Cascade in Hypothalamus Is Beneficial” in Biochemistry and physiology journal by the Omics publishers. Currently Mr. Islam working on a research project entitled “Early Detection of Ovarian Cancer (BRCA1 & BRCA2 Mutation) risk prediction for Bangladesh" the main focus of this project is to develop a risk prediction tool for the ovarian cancer patients in Bangladesh. He has some research publications including PUBMED indexing Journals also.

Abstract: Ebola Hemorrhagic Fever (EHF) has become a public health emergency of international concern which is linear, single stranded, negative strain RNA molecule and has a virally encoded glycoprotein (GP) which was found Zaire in Congo. It is known as zoonotic virus and causes significant morbidity and mortality in primates. Filovirus is transmitted via direct contact with bodily fluids or blood. Treatment of Ebola is not well developed and does not have a possible approved vaccine till now though there are various types of ongoing clinical trials research has been worked out. Since from previous research it shows us that, filovirus is currently now recognized a dangerous threat to the world. Our main objective of this article is to highlight the specific targeted mechanism of actions development for the discovery of anti-Ebola vaccines for the development of treatment strategies to combat Ebola Hemorrhagic Fever.


Suad Alfadhli
Associate Professor, Kuwait University, Kuwait
Title: De-regulation of diabetic regulatory genes in psoriasis: Deciphering the unsolved riddle.

Biography: Suad Alfadhli is currently working as an Associate Professor in the department of medicine, faculty of allied health sciences, Kuwait University, Kuwait. His research interests include PCR Array and the amplification, development of the biomarker genes.

Abstract: The purpose of our study was to identify the currently lacking molecular mechanism that accounts for the co-occurrence of two seemingly disparate diseases: psoriasis and type II diabetes. We aimed to investigate a panel of 84 genes related to the diabetic regulatory network in psoriasis (Ps), psoriasis type II diabetes (Ps-T2D), type II diabetes (T2D) and healthy control (HC). We hypothesize that such attempts would provide novel diagnostic markers and/or insights into pathogenesis of the disease. A quantitative Real Time-PCR Human Diabetes RT(2) Profiler PCR Array was chosen to explore the expression profile 84 diabetic genes in study subjects. Statistical analysis was carried out using appropriate software. The analysis revealed three candidate genes GSK3B, PTPN1, STX4 that are differentially expressed in study subjects. GSK3B was highly significant in Ps-T2D (P=0.00018, FR=-26.6), followed by Ps (P=0.0028, FR=-14.5) and T2D groups (P=0.032, FR=-5.9). PTPN1 showed significant association only with PS-T2D (P=0.00027, FR=-8.5). STX4 showed significant association with both Ps (P=0.0002, FR=-20) and Ps-T2D (P=0.0016, FR=-11.2). ACE represents an additional marker that showed suggestive association with Ps (P=0.0079, FR=-9.37). Our study highlights the complex genetics of Ps-T2D and present biomarkers for the development of T2D in Ps cases.


Samia Faradji-Hamma
Doctoral Researcher, A.Mira university, Algeria
Title: Study of the antibacterial activity of lactic strains isolated from the traditional product with respect to hospital pathogenic strains responsible for urinary infections.

Biography: Dr. Samia Hamma Faradji is a Doctoral researcher at the A.mira University of Béjaia, Algeria. She is also a lecturer in microbiology and a researcher in the laboratory of Applied Microbiology, Milk and probiotics groups. She actively participated in several research projects that deal with the probiotic properties of lactic acid bacteria, technological aspects, and vaporization of traditional products..... She published a book for Master's students entitled: Microbiological analysis techniques.

Abstract: In Algeria urinary infections in children are increasingly important. The resistance of strains isolated from urine to antibiotics involves the search for other substances for the treatment of urinary tract infection. This work deals with the isolation of pathogenic bacteria responsible for urinary infections in 400 children. The study of the antibacterial effect of 30 strains of lactic acid bacteria (BL) against these pathogenic bacteria was carried. The identification of the strains of enterobacterial strains isolated from the urines of 400 older children from 2 to 12 years is carried out by biochemical galleries. The resistance of these strains to antibiotics is studied by antibiogramme. The antibacterial activity of 30 trains of BL selected as preliminary according to their probiotic properties belonging to the five kinds: Lactobacillus , Lactococcus , Leuconostoc, Streptococcus and Pediococcus is studied by the spot and wells test. The identification of isolated strains showed a predominance of E. coli (44.68%) followed by Klebsiella pneumoniae with 29.78%. 30 strains of Enterobacteriaceae were found to be resistant to third generation cephalosporins. E. cloacae are the most resistant species (50%). An image of synergy was observed in 7 strains signifying the production of enzymes of the ESB, this being 3 strains of E. coli, 3 of Klebsiella pneumoniae and 1 strain of Proteus mirabilis. The results of spot test and wells test against the enterobacterial strains show a broad spectrum of activity (18 - 35 mm) of all the strains BL tested. The latter probably produce an active substance of protein nature (protease test). The results obtained can suggested the use of lactic acid bacteria last like an alternative to antibiotics


Navreet Sekhon
Research Scholar, S.G.R.D. Medical College, India
Title: Deciphering the anti-tuberculosis activity of plant extracts and their synergistic effect with widely used anti-tuberculosis drugs.

Biography:

Abstract: Tuberculosis (TB), caused by Mycobacterium tuberculosis. Despite of significant advancement in medical science, it is still one of the leading causes of death and illness worldwide. According to latest WHO global tuberculosis report 2015, approximately 9.6 million people get sick with TB and an estimated 1.6 million died from this disease in 2014 which is even more that HIV/AIDS (1.2 million) (WHO Report, 2015). At present, four first line antibiotics (Rifampicin, Ethambutol, Isoniazid and Pyrazinamide) are being widely used to treat TB under DOTs. Most of the anti TB drugs except streptomycin are reported to cause hepatotoxicity. This is the major cause of discontinuity of DOTs treatment by patients with low income and major factor in emergence of antibiotic resistant strains (MDR and XDR) of M. tuberculosis. Several plant extracts are reported with antimycobacterium activity. Approximately, 60% of the world’s population still relies on medicinal plants for their primary healthcare. Several plant extracts demonstrated antimycobacterium activity. Therefore in the present investigation, we studied the synergistic effect of some plant extracts/phytochemicals along with the Isoniazid and rifampicin. The M. tuberculosis H37Ra was used as model organism. A. vera, A. sativum, A. cepa and A. indica demonstrated the antituberculosis activity. IC50 of antibiotics and plant extracts were determined using resazurine. A. sativum and A. indica demonstrated synergistic effect with isoniazid and rifampicin. Further studies in animal using M.tuberculosis H37Rv are needed to validate these findings.


Phan Van Tan
Faculty of Agriculture and Forestry. Taynguyen University, Vietnam
Title: The determination of total alkaloid, polyphenol, flavonoid and saponine contents in the underground parts of Poganggan (Curcuma sp.)

Biography: Phan Van Tan is currently a biological doctor and a senior lecturer in the Central Highland (Taynguyen) University and he is an executive member of Vietnam Society of Plant Physiology. He teaches Plant Physiology, Stress Physiology, Applied Plant Physiology. His research interests include photosynthesis, phytohormonal application, plant tissue culture, allelopathy, preservation and development of indigenous medicinal plants.

Abstract: Poganggan (Curcuma sp., Zingiberaceae family) was medicinal herb and threatened but it is usually used as a tonic. We successfully micropropagated and cultivated it. This study determined the total alkaloid, polyphenol, flavonoid and saponin content of its growth time in order to determine harvest time. The highest level of total alkaloid content is roots after 11 months (0.272 mg/g), total polyphenol content is rhizomes after 11 months (1.71 mg GAE/g), total flavonoid content is rhizomes after 11 months (13.89 mg QE/g) and total saponin content is roots and rhizomes after 11 months (4.78% and 4.32%). The total content of alkaloid and flavonoid of poganggan is higher than Curcuma zedoaria. The total saponin content of natural pogang gan is equal to 39.5% of 6-year-planted Vietnamese ginseng and the saponin content in rhizomes and tubers of 11-month-planted Pogang gan is from 55.5% to 67.8%, compared with natural Pogang gan.


Jae Woong Lee
Konkuk University, Korea (South)
Title: Biosynthesis of oligomeric anthocyanin from grape skins extracts

Biography: Jae Woong Lee pursued his Bachelor's degree in Biotechnology from Konkuk University. Later he pursued Masters degree in Biotechnology from the Same University. He also published research papers in several international journals.

Abstract: We synthesized the oligomeric anthocyanin from grape skin derived monomeric anthocyanins such as anthocyanidin and proanthocyanidin by fermentation technique using Aspergillus niger, crude enzymes and glucosidase. The biosynthesis of oligomeric anthocyanin was carried out by conventional method using Aspergillus niger and crude enzymes was confirmed by ESI-MS. The molecular weight of the synthesized anthocyanin oligomers was determined using MALDI-MS. Biosynthesis of anthocyanin oligomers using crude enzymes was higher than the synthesis of Aspergillus fermentation. Several studies have been demonstrated that oligomeric anthocyanin has higher antioxidant activity than monomeric anthocyanins. Synthesis of oligomeric anthocyanins is an alternative way of producing efficient anthocyanins that will support the food industries too.


Jae Woong Lee
Konkuk University, Korea (South)
Title: Hydroxyl Radical Scavenging Activity and Protective Effect of Biosynthesized Oligomeric Anthocyanin on t-BHP-Triggered G2/M Arrest in Retinal Cells

Biography: Jae Woong Lee pursued his Bachelor's degree in Biotechnology from Konkuk University. Later he pursued Masters degree in Biotechnology from the Same University. He also published research papers in several international journals.

Abstract: In this study, the free-radical-scavenging activities of oligomeric anthocyanin on hydroxyl radicals were evaluated using electron spin resonance (ESR) spectroscopy. The hydroxyl radical scavenging activity of oligomeric anthocyanin was increased in a dose-dependent manner, with 0.28 mg/mL half maximal inhibitory concentration value (IC50). In addition, oligomeric anthocyanin inhibited the t-BHP-induced G2/M phase cell cycle arrest by modulating the expression levels of p21, cdc2, p53 and cyclin B1 proteins in ARPE-19 cells. Taken together, the present study results demonstrate that oligomeric anthocyanins have high antioxidative activity and they can regulate cell cycle arrest


Yon-Suk Kim
Research Scholar, Konkuk University, Korea (South)
Title: Anti-oxidant activity of Lindera glaucabark extracts

Biography: Dr. Yon-Suk Kim is currently working as a postdoctoral fellow in the Department of Biotechnology, Konkuk University, Chungju, South Korea. Her area of research interest is to elucidate the role of bioactive compounds found from the bio-resources. She received her Ph. D degree with research of antioxidant and anti-inflammatory effect of plant extracts and their bioactive compounds.

Abstract: Lindera glauca (Lauraceae), a pretty and small deciduous tree, is extensively distributed in the mountainous districts of China, Japan and Korea. However, there are few studies on antioxidant activity of Lindera glauca extracts. The study was aimed at determining the antioxidant activity, the contents of polyphenol and flavonoid of Lindera glauca bark extracts (LGB). Various assays were employed to evaluate the antioxidant property of water and ethanol extracts from the LGB,compared to those of the other natural and syntheticantioxidants. LGB extracts had high total phenolic contentsin both the water (98.04 ± 2.56 mg GAE/g extracts) andethanol extracts (105.61 ± 1.44 mg GAE/g extracts). In addition, total flavonoid contents were high in both the water extracts (44.48 ± 0.44 mg CE/g extracts) and ethanol extracts (63.97 ± 2.35 CE/g extracts). In addition, antioxidant capacity in ferric reducing antioxidant power (FRAP) of the water and ethanolic extracts from LGB were 1.020±0.031 and 1.169±0.049 mM FeSO4 equivalent/mg extract, respectively. The ABTS radical scavenging activities of the water and ethanolic extracts from LGB were 0.833±0.003 and 0.932±0.001 mM Trolox equivalent/mg extract, respectively.The antioxidant activity of extracts from LGB showed stronger activity than those of vitamin C and α-tocopherol in ferric thiocyanate (FTC) and thiobarbituric acid (TBA) methods.These results indicate that the LGB extracts possessed antioxidant activity


Prof Mohamad Zouheir Habbal
Professor, American University of Beirut, Lebanon
Title: The clinical chemistry of circulating white blood cells ( WBC) : A new paradigm

Biography: Dr. Mohamad Zouheir Habbal is currently a Professor at American University of Beirut Medical Center, Lebanon. He completed his B.S in pharmacy from the Damascus University, M.S and Ph. D in Biochemistry from the American University of Beirut Medical Center, Lebanon. He is a recipient of many positions and published many books, articles and the abstracts. He has a membership in many professional organizations.

Abstract: Recent advances in Biotechnology made it possible the analysis of a large number of serum analytes in a very small volume ( 30 analytes in 100nl ).To investigate patients with inborn errors of metabolism, certain tests require an enzyme assay that is found in circulating WBC. Following WBC isolation, purification and sonication, the enzyme of interest level in the clear supernate is assayed. We have used that clear supernate to measure serum-specific analytes. In spite of very low concentration of proteins ( less than 3.0 mg /ml) , some analytes are found in high concentration ,compared to serum ( calculated As units or mg per unit or mg in serum), such as ALT, LDH, ferritin, folic acid. Others are totally new such As thyroid hormones. Still others, are totally absent such as albumin,amylase and creatinine. Similar Results were obtained on different samples. Again advances in Biotechnology made it possible to cell-culture various types of tissues, whether two Dimensional or recently threesome, the clinical chemistry of lysates of these cells is possible which Represents a new paradigm. Definitely, this analysis would have a huge impact on the biology of such cells derived from different Tissues normal or abnormal in health and disease.


Aisha Tahir
Research Scholar, University of Agriculture, Pakistan
Title: Evaluation of Molecular based Conservation Tool by Comparing rbcLa and ITS2 as DNA barcode regions

Biography: Aisha Tahir is currently a PhD research scholar in Biochemistry in the department of Biochemistry of University of Agriculture, Faisalabad Pakistan. She has completed her M.Phil in Biochemistry working on molecular identification of different fungal species. She is currently working on medicinal plants of Pakistan as her PhD research project. Her research interests focus on exploring and evaluating molecular based methods of species identification of plants, animals and microbes in combination of using bioinformatics tools. She has training and work experience on DNA barcoding of plants and animals in Biodiversity Institute of Ontario, University of Guelph, Canada and Canadian Centre for DNA Barcoding.

Abstract: Reference DNA barcode resource for selected indigenous medicinal plants of Pakistan was generated in the current project that covered the most commonly used medicinal plants (130 species) belonged to different areas of Pakistan. The potential of DNA barcoding was tested on diverse taxa of medicinal plants by using core plastid gene regionrbcLa and a nuclear region ITS2 as barcode regions. Vouchers and metadata of 258 specimens (triplicate of all species) were up loaded on BOLD with project name of "DNA Barcoding of Medicinal Plants of Pakistan (DBMPP)" after morphological identification. The success rates of the amplification of two DNA barcoding markers (rbcLa and ITS2) were 96% and 82% respectively using single primer pairs for each marker. Sequencing success rates were 100% (rbcLa) and 98% (ITS2). Gene region statistics were used as pre-screening test of DNA barcode sequences that revealed that ITS2 could be chosen as DNA barcode region for a dataset of diverse taxa. Mean pairwise distance, near neighbor analysis, best close match and BLASTn all methods of evaluation supported the hypothesis that ITS2 has the best potential to be used as barcode region for plants but have few limitations of amplification difficulty and alignment after sequencing. Combination ofrbcLa+ITS2 was also tested that didn't improve the identification of species significantly. Method of BLASTn concluded that ITS2assigned 99% samples (known to database) to species level whilerbcLaassigned 78% to species level but it requires building comprehensive and authentic sequence libraries for sequence comparison beforehand. The same results were pictured in distance based Neighbor Joining tree. Total 621 sequences were generated in this study with 312 rbcLaand 309 ITS2. The practical utility of the region wise barcode sequence database is in the fields of health and medicine (herbal drug quality control), forensics, economy (to check illegal trade of threatened species) and conservation biology.


Sidibe El Hassane
Associate Professor, college de medecine des hopitaux de paris, Senegal
Title: THYROID GLAND AND ITS PEPTIDES OR ACTUALITY OF GENERAL ENDOCRINOLOGY

Biography:

Abstract: Many peptides by their cell molecular metabolism in the thyroid gland are involved: mutations of TSH receptor is linked to hormone resistance with congenital hypothyroidism. ; A new oroxigenic peptide, Ghrelin is widespread in endocrine tissues; tachykinine is another novel peptide with endocrine paracrine effect. PTTG and FGF-2 are prognostic markers of differentiated cancers ; CRIF1 is a novel protein of nucleus interacting with Gadd45 and could negatively regulate cycle cell progression and cell growth ; mutations of RET proto oncogene is now the best CMT biomarker in the place of calcitonin in addition with CGRP, CGRPβ and Amylin ; regulating proteins of CAMP, protein G, Gi alpha 1, MAL proteolipid are identified near immunologic antigens involved in Grave’s disease, autoimmune polyendocrinopathies. These peptides by their diversity of function support the concept of general endocrinology equivalent of medical sciences.


Beong Ou Lim
Assistant Professor, Konkuk University, Korea (South)
Title: Apigenin Attenuates Melanoma Cell Migration by Inducing Anoikis through Integrin and Focal Adhesion Kinase Inhibition

Biography: Beoung Ou Lim is an assistant professor at department of life science in konkuk University, glocal campus, South Korea. He earned his master of science from Kyushu University in 1994 and achieved his PhD in Food Chemical Engineering from the same university in the year of 1997. During his PhD he did some noticeable work. Currently he has over 50 publications as a corresponding author. His recent publications include “Preventive and therapeutic effects of blueberry extract against DSS-induced ulcerative colitis regulation of antioxidant and inflammatory mediators,” His current research interest includes the food medical bio-compound.

Abstract: Apigenin, a nonmutagenic flavonoid, has been found to have antitumor properties and is therefore particularly relevant for the development of chemotherapeutic agents for cancers. In this study, time- and dose-dependent cell viability and cytotoxicity were assessed to determine the effects of apigenin on A2058 and A375 melanoma cells. Melanoma cells were pretreated with different concentrations of apigenin and analyzed for morphological changes, anoikis induction, cell migration, and levels of proteins associated with apoptosis. Apigenin reduced integrin protein levels and inhibited the phosphorylation of focal adhesion kinase (FAK) and extracellular signal-regulated kinase (ERK1/2), which induce anoikis in human cutaneous melanoma cells. Apigenin exhibited dose-dependent inhibition of melanoma cell migration, unlike untreated controls. Furthermore, apigenin treatment increased apoptotic factors such as caspase-3 and cleaved poly(ADP-ribose) polymerase in a dose-dependent manner, demonstrating the metastasis of melanoma cells. Our results provide a new insight into the mechanisms by which apigenin prevents melanoma metastasis by sensitizing anoikis induced by the loss of integrin proteins in the FAK/ERK1/2 signaling pathway. These findings elucidate the related mechanisms and suggest the potential of apigenin in developing clinical treatment strategies against malignant melanoma.


ICBB-2017| by: Scientific Future Group